{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T19:10:37Z","timestamp":1780341037455,"version":"3.54.1"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2016,8,1]],"date-time":"2016-08-01T00:00:00Z","timestamp":1470009600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"publisher","award":["81502787"],"award-info":[{"award-number":["81502787"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"publisher","award":["21276071"],"award-info":[{"award-number":["21276071"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Hunan Provincial Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12JJ5005"],"award-info":[{"award-number":["12JJ5005"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Postdoctoral Funding","award":["2015M572274"],"award-info":[{"award-number":["2015M572274"]}]},{"name":"Open Funding","award":["2014EME001"],"award-info":[{"award-number":["2014EME001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>A novel magnetic targeting anti-tumour drug delivery system (Fe3O4\/KCTS-CHE) was designed using magnetic Fe3O4\/chitosan alpha-ketoglutaric acid (Fe3O4\/KCTS) as carrier and chelerythrine (CHE) as an anti-tumour drug model. Moreover, the anti-tumour activities and mechanisms of Fe3O4\/KCTS-CHE were investigated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The preparation conditions of Fe3O4\/KCTS-CHE microspheres were optimized by response surface methodology (RSM). The CHE drug release kinetics was evaluated by fitting the experimental data to standard release equations. The inhibitive activities of Fe3O4\/KCTS-CHE microspheres against the HepG2 cells were estimated using MTT assay in vitro, and the mechanisms were studied using Hoechst 33258 staining.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The optimum preparation conditions were 11.68 : 1 for Fe3O4\/KCTS:CHE ratio, 4 : 1 for oil\/water ratio and 50.03 min for the ultrasonic time. The drug loading content and entrapment efficiency under the optimal conditions were 23.3% and 50.9%. The best fit was Higuchi model for the microspheres. The inhibitive rate on HepG2 cells of Fe3O4\/KCTS-CHE nanoparticles varied from 30.19 \u00b1 2.64% to 70.46 \u00b1 6.42% at different concentrations from 50 to 400 mg\/l in 72 h.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Fe3O4\/KCTS-CHE exhibited effective anti-tumour activities against the HepG2 cells and induced cell apoptosis in HepG2 cells. Fe3O4\/KCTS-CHE possess a high drug loading efficiency and entrapment efficiency, which are a new matrix for controlling release of drugs and a promising candidate for targeted drug delivery.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jphp.12564","type":"journal-article","created":{"date-parts":[[2016,6,13]],"date-time":"2016-06-13T01:26:45Z","timestamp":1465781205000},"page":"1030-1040","source":"Crossref","is-referenced-by-count":6,"title":["Optimization of a novel chelerythrine-loaded magnetic Fe3O4\/chitosan alpha-ketoglutaric acid system and evaluation of its anti-tumour activities"],"prefix":"10.1093","volume":"68","author":[{"given":"Fei","family":"Yang","sequence":"first","affiliation":[{"name":"School of Public Health, Central South University , Changsha,","place":["China"]},{"name":"Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University , 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