{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:59:49Z","timestamp":1761663589618,"version":"3.37.3"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>This study was designed to evaluate the antihyperglycaemic activity of the methanol leaf extract of Eremophila maculata (EMM) both in vitro and in vivo.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The antihyperglycaemic activity was assessed in vitro using differentiated 3T3-L1 adipocytes, whereas in-vivo effect was evaluated in streptozotocin-induced diabetic rats. Chemical profiling of EMM was done using LC-ESI-MS techniques. Molecular modelling experiments of the identified compounds were performed using C-Docker protocol.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>Eremophila maculata slightly enhanced cellular glucose uptake and utilization in vitro by 3.92% relative to the untreated control. A stronger in-vivo effect was observed for EMM and its dichloromethane fraction. A pronounced elevation in serum insulin by 88.89 and 66.67%, respectively, accompanied by an apparent decline in fasting blood glucose (FBG) level by 65.60 and 70.37% comparable to streptozotocin-induced diabetic rats was observed. This effect was stronger than that of the reference drug glibenclamide (GLB). Chemical profiling of EMM revealed that leucoseptoside A, verbascoside, syringaresinol-4-O-\u03b2-D-glucopyranoside, pinoresinol-4-O-\u03b2-D-glucopyranoside and pinoresinol-4-O-[6\u2033-O-(E)-feruloyl]-\u03b2-D-glucopyranoside are the major compounds. Molecular modelling showed that martynoside, verbascoside and phillygenin exhibited the highest inhibition to human pancreatic \u03b1-amylase (HPA), maltase glucoamylase (MGAM) and aldose reductase (AR), respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Eremophila maculata offers an interesting relatively safer antihyperglycaemic candidate comparable to synthetic analogues.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jphp.12690","type":"journal-article","created":{"date-parts":[[2017,3,21]],"date-time":"2017-03-21T18:40:22Z","timestamp":1490121622000},"page":"733-742","source":"Crossref","is-referenced-by-count":35,"title":["Antihyperglycaemic activity of the methanol extract from leaves of <i>Eremophila maculata<\/i> (Scrophulariaceae) in streptozotocin-induced diabetic rats"],"prefix":"10.1093","volume":"69","author":[{"given":"Fadia S","family":"Youssef","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University , Cairo,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9270-6267","authenticated-orcid":false,"given":"Mohamed L","family":"Ashour","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University , Cairo,","place":["Egypt"]},{"name":"Institute of Pharmacy and Molecular Biotechnology, Heidelberg University , Heidelberg,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherif S","family":"Ebada","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University , Cairo,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mansour","family":"Sobeh","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Molecular Biotechnology, Heidelberg University , Heidelberg,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hesham A","family":"El-Beshbishy","sequence":"additional","affiliation":[{"name":"Medical Laboratory Sciences Department, Fakeeh College for Medical Sceinces , Jeddah,","place":["Saudi Arabia"]},{"name":"Biochemistry Department, Faculty of Pharmacy, Al-Azhar University , Cairo,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdel Nasser","family":"Singab","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University , Cairo,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Wink","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Molecular Biotechnology, Heidelberg University , Heidelberg,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2017,6,1]]},"reference":[{"key":"2024103012573575000_jphp12690-cit-0001","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.jep.2003.09.034","article-title":"Ethanolic extract of Ocimum sanctum leaves partially attenuates streptozotocin-induced alterations in glycogen content and carbohydrate metabolism in rats","volume":"90","author":"Vats","year":"2004","journal-title":"J Ethnopharmacol"},{"key":"2024103012573575000_jphp12690-cit-0002","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1211\/0022357044607","article-title":"Antihyperglycaemic and antioxidant effect of hyponidd, an ayurvedic herbomineral formulation in streptozotocin-induced diabetic rats","volume":"56","author":"Babu","year":"2004","journal-title":"J Pharm Pharmacol"},{"key":"2024103012573575000_jphp12690-cit-0003","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1211\/jpp.58.8.0010","article-title":"The influence of rutin on the extracellular matrix in streptozotocin-induced diabetic rat kidney","volume":"58","author":"Kamalakkannan","year":"2006","journal-title":"J Pharm Pharmacol"},{"key":"2024103012573575000_jphp12690-cit-0004","first-page":"151","article-title":"Medicinal plants with potential antidiabetic activity and their assessment","volume":"3","author":"Singab","year":"2014","journal-title":"Med Aromat Plants"},{"key":"2024103012573575000_jphp12690-cit-0005","first-page":"265","article-title":"Anti-diabetic activity of Tinospora cordifolia (Willd.) in streptozotocin diabetic rats; 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