{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T19:42:17Z","timestamp":1768765337642,"version":"3.49.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["lzujbky-2013-154"],"award-info":[{"award-number":["lzujbky-2013-154"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Foundation of Key Laboratory for Gastrointestinal Diseases of Gansu Province","award":["gswcky-2012-006"],"award-info":[{"award-number":["gswcky-2012-006"]}]},{"name":"Health Industry Research Project of Gansu Province","award":["GWGL2014-54"],"award-info":[{"award-number":["GWGL2014-54"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Chimeric peptide MCRT, based on morphiceptin and PFRTic-NH2, was a bifunctional ligand of \u03bc- and \u03b4-opioid receptors (MOR-DOR) and produced potent analgesia in tail-withdrawal test. The study focused on the supraspinal effects of morphiceptin, PFRTic-NH2 and MCRT on gastrointestinal motility. Moreover, opioid receptor antagonists, naloxone (non-selective), cyprodime (MOR selective) and naltrindole (DOR selective) were utilized to explore the mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Intracerebroventricular administration was achieved via the implanted cannula. Gastric emptying and intestinal transit were measured to evaluate gastrointestinal motility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key findings<\/jats:title><jats:p>(1) At supraspinal level, morphiceptin, PFRTic-NH2 and MCRT significantly decreased gastric emptying and intestinal transit; (2) MCRT at 1 nmol\/mouse, far higher than its analgesic dose (ED50 = 29.8 pmol\/mouse), failed to regulate the gastrointestinal motility; (3) MCRT-induced gastrointestinal dysfunction could be completely blocked by naloxone and naltrindole, but not affected by cyprodime.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>(1) Morphiceptin and PFRTic-NH2 played important roles in the regulation of gastrointestinal motility; (2) MCRT possessed higher bioactivity of pain relief than gastrointestinal regulation, suggesting its promising analgesic property; (3) MCRT-induced motility disorders were sensitive to DOR but not to MOR blockade, indicating the pain-relieving specificity of speculated MOR subtype or splice variant or MOR-DOR heterodimer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jphp.12761","type":"journal-article","created":{"date-parts":[[2017,6,16]],"date-time":"2017-06-16T06:32:38Z","timestamp":1497594758000},"page":"1244-1251","source":"Crossref","is-referenced-by-count":9,"title":["Supraspinal inhibitory effects of chimeric peptide MCRT on gastrointestinal motility in mice"],"prefix":"10.1093","volume":"69","author":[{"given":"Chunbo","family":"He","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University , Lanzhou,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailan","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University , 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