{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:23:57Z","timestamp":1758925437695,"version":"3.37.3"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"PAICYT","award":["SA196-15"],"award-info":[{"award-number":["SA196-15"]}]},{"DOI":"10.13039\/501100004685","name":"UANL","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004685","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PRODEP"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>A cell-penetrating peptide-based delivery system could target specific types of cells for therapeutic genes delivery. To increase the gene delivery efficiency into neuronal phenotype cells, we introduced an Asn194Lys mutation to RVG29 peptide derived from rabies virus glycoprotein and added a nuclear localization signal to enhance its nuclear import.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Mutant RVG or wild-type RVG peptide, a karyophilic peptide (KP) and a plasmid encoding green fluorescent protein (pGL) were bound by electrostatic charges to form four different kinds of RVG complexes. Immunofluorescence was used to assess the gene transfection efficiency into astrocytes, oligodendrocyte precursor cells (OPCs), SH-SY5Y, HeLa and NIH\/3T3 cells. The cellular uptake mechanism of RVG29 complexes was examined using endocytosis inhibitors.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The mRVG29 peptide has the ability to enhance the nuclear import of plasmids. The Asn194Lys mutation in RVG29 peptide of the pGL-mRVG29 complex and the addition of KP to the pGL-RVG29-KP complex increased the capacity to deliver DNA by endocytosis in astrocytes and SH-SY5Y cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The complexes pGL-mRVG29 and pGL-RVG29-KP have specificity for transfecting astrocytes and SH-SY5Y cells. The karyophilic capacity of this new mRVG peptide render it promising candidate to act as gene delivery vector into the brain cells.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jphp.12766","type":"journal-article","created":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T09:53:17Z","timestamp":1498211597000},"page":"1352-1363","source":"Crossref","is-referenced-by-count":11,"title":["Asn194Lys mutation in RVG29 peptide increases GFP transgene delivery by endocytosis to neuroblastoma and astrocyte cells"],"prefix":"10.1093","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8983-3620","authenticated-orcid":false,"given":"Sheila Adela","family":"Villa-Cedillo","sequence":"first","affiliation":[{"name":"Departamento de Histolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey,","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto","family":"Rodr\u00edguez-Rocha","sequence":"additional","affiliation":[{"name":"Departamento de Histolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey,","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Mireya","family":"Zavala-Flores","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular, Instituto Mexicano del Seguro Social, Centro de Investigaci\u00f3n Biom\u00e9dica del Noreste , Monterrey,","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Montes-de-Oca-Luna","sequence":"additional","affiliation":[{"name":"Departamento de Histolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey,","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aracely","family":"Garc\u00eda-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Histolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey,","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria de Jesus","family":"Loera-Arias","sequence":"additional","affiliation":[{"name":"Departamento de Histolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey,","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odila","family":"Saucedo-C\u00e1rdenas","sequence":"additional","affiliation":[{"name":"Departamento de Histolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Nuevo Le\u00f3n , Monterrey,","place":["Mexico"]},{"name":"Departamento de Gen\u00e9tica Molecular, Instituto Mexicano del Seguro Social, Centro de 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