{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:05:10Z","timestamp":1740135910861,"version":"3.37.3"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Science and Technology Bureau of Chenzhou, Hunan Province, China","award":["jsyf2017025"],"award-info":[{"award-number":["jsyf2017025"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81202535","81470493"],"award-info":[{"award-number":["81202535","81470493"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Fund Project of Hunan Provincial Science and Technology Department","award":["2018JJ6097"],"award-info":[{"award-number":["2018JJ6097"]}]},{"name":"unan Provincial Health and Family Planning Commission funded projects","award":["2017183"],"award-info":[{"award-number":["2017183"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The current study aimed to explore whether minimally modified low-density lipoprotein (mmLDL) via tail vein injection upregulates the ETB and \u03b11 receptors in mouse mesenteric arteries by activating the PI3K\/Akt pathway.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The contraction curves of the mesenteric arteries caused by sarafotoxin 6c (S6c, ETB receptor agonist) and phenylephrine (PE, \u03b11 receptor agonist) were measured by a myograph system. Serum oxLDL was detected using enzyme-linked immunosorbent assays. The levels of the ETB receptor, the \u03b11 receptor, PI3K, p-PI3K and p-Akt were detected using real-time polymerase chain reaction and Western blot analyses.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>Minimally modified low-density lipoprotein noticeably enhanced the contraction effect curves of S6c and PE, with significantly increased Emax values (P &amp;lt; 0.01), compared to those of the control group. This treatment significantly increased the mRNA expression and protein levels of the ETB and \u03b11 receptors and the protein levels of p-PI3K and p-Akt in the vessel wall (P &amp;lt; 0.01). LY294002 inhibited the effect of mmLDL.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>An increase in mmLDL activated the PI3K\/Akt pathway, which upregulated the expression of the ETB and \u03b11 receptors and enhanced the ETB and \u03b11-receptor-mediated contractile function.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jphp.13069","type":"journal-article","created":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T06:28:08Z","timestamp":1548052088000},"page":"937-944","source":"Crossref","is-referenced-by-count":1,"title":["Minimally modified low-density lipoprotein upregulates the ETB and \u03b11 receptors in mouse mesenteric arteries in vivo by activating the PI3K\/Akt pathway"],"prefix":"10.1093","volume":"71","author":[{"given":"Zhong-San","family":"Zeng","sequence":"first","affiliation":[{"name":"Institute of Pharmacy and Pharmacology, The First People's Hospital of Chenzhou, University of South China , Chenzhou,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Pharmacology, The First People's Hospital of Chenzhou, University of South China , Chenzhou,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cang-Bao","family":"Xu","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Institute of Basic and Translational Medicine, Xi'an Medical University , Xi'an,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center , Xi'an,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Pharmacology, The First People's Hospital of Chenzhou, University of South China , Chenzhou,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9411-6623","authenticated-orcid":false,"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Pharmacology, The First People's Hospital of Chenzhou, University of South China , Chenzhou,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,6,1]]},"reference":[{"key":"2024103012322289600_jphp13069-cit-0001","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.atherosclerosis.2012.07.039","article-title":"Pentameric CRP attenuates inflammatory effects of mmLDL by 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