{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:53:22Z","timestamp":1788951202572,"version":"build-2803163510"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100010877","name":"Shenzhen Science and Technology Innovation Committee","doi-asserted-by":"crossref","award":["JCYJ20160428180724294"],"award-info":[{"award-number":["JCYJ20160428180724294"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To investigate the potential role and mechanism of TUPS, a soluble epoxide hydrolase inhibitor, in cardiac hypertrophy.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Rat and H9C2 cell models of cardiac hypertrophy were induced by isoproterenol and angiotensin II, respectively, followed by TUPS treatment. The expression of hypertrophic markers, ANP and BNP, was determined by quantitative real-time PCR. The abundance of Beclin-1, LC3, p-AMPK and phosphorylated-mammalian target of rapamycin (p-mTOR) proteins was analysed by Western blot and immunohistocytology. Cell morphology and viability were evaluated by F-actin staining and MTS. H9C2 cells were transfected with GFP-LC3 to evaluate autophagy flux.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>TUPS significantly inhibited rat heart size, heart weight-to-body weight ratio, heart wall thickness, hypertrophic H9C2 cell swelling and viability suppression as well as the expression of ANP and BNP genes in hypertrophic models. In addition, autophagic markers Beclin-1 and LC3 were elevated in both cellular and animal models, which were suppressed by TUPS, with corresponding changes of autophagy flux. The abundance of p-AMPK was increased, while p-mTOR was decreased in hypertrophic cells, which were abolished by TUPS. Rapamycin decreased p-mTOR level, increased Beclin-1 and LC3 expression and induced cell size enlargement and cell viability inhibition in hypertrophic H9C2 cells treated with TUPS.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>TUPS inhibits cardiac hypertrophy by regulating mTOR\/autophagy axis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jphp.13113","type":"journal-article","created":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T09:47:31Z","timestamp":1560937651000},"page":"1291-1300","source":"Crossref","is-referenced-by-count":8,"title":["Soluble epoxide hydrolase inhibitor, TUPS, attenuates isoproterenol\/angiotensin II-induced cardiac hypertrophy through mammalian target of rapamycin-mediated autophagy inhibition"],"prefix":"10.1093","volume":"71","author":[{"given":"Huanji","family":"Zhang","sequence":"first","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-sen University , Shenzhen,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sun Yat-sen Memorial Hospital, Sun Yat-sen University , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwen","family":"Liang","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-sen University , Shenzhen,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Yan","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-sen University , Shenzhen,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fensheng","family":"Wu","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-sen University , Shenzhen,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenmin","family":"Xu","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-sen University , Shenzhen,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwen","family":"Wu","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-sen University , Shenzhen,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixi","family":"Chen","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-sen University , Shenzhen,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongquan","family":"Pan","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-sen University , 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