{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T17:51:09Z","timestamp":1759341069120,"version":"3.37.3"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["CBB-APQ-2008-12"],"award-info":[{"award-number":["CBB-APQ-2008-12"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title\/>\n                  <jats:p>The alkylaminoalkanethiosulfuric acids (AAATs) are amphipathic compounds effective against experimental schistosomiasis, of low toxicity, elevated bioavailability after a single oral dose and prompt tissue absorption.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To explore the in-vitro antileishmanial potential of AAATs using five compounds of this series against Leishmania (Viannia) braziliensis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Their effects on promastigotes and axenic amastigotes, and cytotoxicity to macrophages were tested by the MTT method, and on Leishmania-infected macrophages by Giemsa stain. Effects on the mitochondrial membrane potential of promastigotes and axenic amastigotes and DNA of intracellular amastigotes were tested using JC-1 and TUNEL assays, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The 2-(isopropylamino)-1-octanethiosulfuric acid (I) and 2-(sec-butylamino)-1-octanethiosulfuric acid (II) exhibit activity against both promastigotes and intracellular amastigotes (IC50 25-35 \u00b5m), being more toxic to intracellular parasites than to the host cell. Compound I induced a loss of viability of axenic amastigotes, significantly reduced (30%) the mitochondrial membrane potential of both promastigotes and axenic amastigotes and promoted selective DNA fragmentation of the nucleus and kinetoplast of intracellular amastigotes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In this previously unpublished study of trypanosomatids, it is shown that AAATs could also exhibit selective antileishmanial activity, a new possibility to be investigated in oral treatment of leishmaniasis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jphp.13163","type":"journal-article","created":{"date-parts":[[2019,10,3]],"date-time":"2019-10-03T10:04:05Z","timestamp":1570097045000},"page":"1784-1791","source":"Crossref","is-referenced-by-count":6,"title":["The alkylaminoalkanethiosulfuric acids exhibit <i>in-vitro<\/i> antileishmanial activity against <i>Leishmania (Viannia) braziliensis<\/i>: a new perspective for use of these schistosomicidal agents"],"prefix":"10.1093","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3407-3123","authenticated-orcid":false,"given":"Gabriane Nascimento","family":"Porcino","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora , Juiz de Fora,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana Maria Ribeiro","family":"Antinarelli","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora , Juiz de Fora,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina Ribeiro Gomes","family":"Maia","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora , Juiz de Fora,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila","family":"Faria-Pinto","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora , Juiz de Fora,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Taunay-Rodrigues","sequence":"additional","affiliation":[{"name":"Faculdade Princesa do Oeste , Crat\u00e9us,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Marcos","family":"Zech Coelho","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz , Belo Horizonte,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David Lee","family":"Nelson","sequence":"additional","affiliation":[{"name":"Pro-Reitoria de Pesquisa e P\u00f3s-Gradua\u00e7\u00e3o, Universidade Federal dos Vales de Jequitinhonha e Mucuri , Diamantina,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus Luiz Oliveira","family":"Penido","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora , Juiz de Fora,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1005-278X","authenticated-orcid":false,"given":"Elaine Soares","family":"Coimbra","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Juiz de Fora , Juiz de Fora,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eveline 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