{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:13:15Z","timestamp":1787015595256,"version":"build-2736575974"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to explore the pharmacological properties of pinoresinol-4-O-\u03b2-D-glucopyranoside (PG), isolated from prunes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In-vitro antioxidant activity was assessed using ferric reducing antioxidant power (FRAP) and 2,2'-azino-bis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS) assays. In-vivo hepatoprotective activity was evaluated using CCl4-induced hepatotoxicity mouse model. The antihyperglycaemic activity was determined in vitro using \u03b1-glucosidase and \u03b1-amylase inhibiting activity and in vivo using streptozotocin-treated model. Molecular modelling was done on \u03b1-amylase, \u03b1-glucosidase, aldose reductase and peroxisome proliferator-activated receptor gamma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key findings<\/jats:title>\n                    <jats:p>Pinoresinol-4-O-\u03b2-D-glucopyranoside showed promising antioxidant activity in FRAP and ABTS assays with total antioxidant capacity equal 418.47 and 1091.3 \u00b5mol\/g in terms of ascorbic acid, respectively. PG (50 mg\/kg b.w.) exhibited a hepatoprotective activity in vivo as it lowered AST and ALT levels. PG showed a potent in-vitro antihyperglycaemic activity as it inhibited \u03b1-glucosidase with an IC50 value of 48.13 \u03bcg\/ml. PG caused a prominent decline in serum glucose level by 37.83% in streptozotocin-treated mice with promising elevation in insulin level of 25.37%. Oxidative stress markers were reduced by PG, and it showed a high fitting on \u03b1-amylase and \u03b1-glucosidase active sites.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Pinoresinol-4-O-\u03b2-D-glucopyranoside is a natural entity combating oxidative stress, hepatic damage and diabetes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jphp.13358","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T10:27:16Z","timestamp":1598610436000},"page":"1830-1839","source":"Crossref","is-referenced-by-count":46,"title":["Pinoresinol-4-\n                    <i>O<\/i>\n                    -\n                    <i>\u03b2<\/i>\n                    -D-glucopyranoside: a lignan from prunes (\n                    <i>Prunus domestica<\/i>\n                    ) attenuates oxidative stress, hyperglycaemia and hepatic toxicity\n                    <i>in vitro<\/i>\n                    and\n                    <i>in vivo<\/i>"],"prefix":"10.1093","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5871-2639","authenticated-orcid":false,"given":"Fadia S","family":"Youssef","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University , Cairo,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9270-6267","authenticated-orcid":false,"given":"Mohamed L","family":"Ashour","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University , Cairo,","place":["Egypt"]},{"name":"Batterjee Medical College , Jeddah,","place":["Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4357-0981","authenticated-orcid":false,"given":"Hesham A","family":"El-Beshbishy","sequence":"additional","affiliation":[{"name":"Medical Laboratory Sciences Department, Fakeeh College for Medical Sciences , Jeddah,","place":["Saudi Arabia"]},{"name":"Biochemistry Department, Faculty of Pharmacy, Al-Azhar University , Cairo,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5450-1116","authenticated-orcid":false,"given":"Alaaeldin","family":"Ahmed Hamza","sequence":"additional","affiliation":[{"name":"Hormone Evaluation Department, National Organization for Drug Control and Research (NODCAR) , Giza,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7445-963X","authenticated-orcid":false,"given":"Abdel Nasser B","family":"Singab","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University , Cairo,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7875-4510","authenticated-orcid":false,"given":"Michael","family":"Wink","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy and Molecular Biotechnology, Heidelberg University , Heidelberg,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,12,1]]},"reference":[{"key":"2024110604205477100_jphp13358-cit-0001","first-page":"80","article-title":"The antiproliferative effect of Origanum majorana on human hepatocarcinoma cell line: suppression of NF-kB","volume":"62","author":"Fathy","year":"2016","journal-title":"Cell Mol Biol"},{"key":"2024110604205477100_jphp13358-cit-0002","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.indcrop.2018.03.034","article-title":"New flavonoid glycosides from two Astragalus species (Fabaceae) and validation of their 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