{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:49:51Z","timestamp":1783579791589,"version":"3.55.0"},"reference-count":69,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T00:00:00Z","timestamp":1358380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2013,4]]},"abstract":"Abstract<\/jats:title>In humans, two main types of membrane melatonin receptors have been identified, MT<\/jats:styled-content>1 and MT<\/jats:styled-content>2. Expression of MT<\/jats:styled-content>1 in neoplastic cells seems to increase the efficacy of melatonin's oncostatic activity. The purpose of this study was to determine the distribution and the intensity of MT<\/jats:styled-content>1 expression in breast cancer cells and to correlate it with clinicopathological factors. Immunohistochemical studies (IHC<\/jats:styled-content>) were conducted on 190 cases of invasive ductal breast carcinomas (IDC<\/jats:styled-content>) and molecular studies were performed on 29 cases of frozen tumor fragments and selected breast cancer cell lines. Most of the studied tumors manifested a membranous\/cytoplasmic IHC<\/jats:styled-content> expression of MT<\/jats:styled-content>1. In IDC<\/jats:styled-content>, the MT<\/jats:styled-content>1 expression was higher than in fibrocystic breast disease. MT<\/jats:styled-content>1 expression was higher in estrogen receptor positive (ER<\/jats:styled-content>+) and HER<\/jats:styled-content>2 positive (HER<\/jats:styled-content>2+) tumors. Triple negative tumors (TN<\/jats:styled-content>) manifested the lowest MT<\/jats:styled-content>1 expression level. The lowest MT<\/jats:styled-content>1 protein expression level was noted in the TN<\/jats:styled-content> breast cancer cell line MDA<\/jats:styled-content>\u2010MB<\/jats:styled-content>\u2010231 compared with ER<\/jats:styled-content>+ cell lines MCF<\/jats:styled-content>\u20107 and SK<\/jats:styled-content>\u2010BR<\/jats:styled-content>\u20103. MT<\/jats:styled-content>1 mRNA<\/jats:styled-content> expression was negatively correlated with the malignancy grade of the studied IDC<\/jats:styled-content> cases. Moreover, higher MT<\/jats:styled-content>1 expression was associated with patients' longer overall survival (OS<\/jats:styled-content>) in the group of ER<\/jats:styled-content>+ breast cancers and treated with tamoxifen. Multivariate analysis indicated that MT<\/jats:styled-content>1 was an independent prognostic factor in the ER<\/jats:styled-content>+ tumors for OS<\/jats:styled-content> and event\u2010free survival in the ER<\/jats:styled-content>+ tumors. The results of this study may point to a potential prognostic and therapeutic significance of MT<\/jats:styled-content>1 in IDC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jpi.12032","type":"journal-article","created":{"date-parts":[[2012,12,13]],"date-time":"2012-12-13T20:11:55Z","timestamp":1355429515000},"page":"334-345","source":"Crossref","is-referenced-by-count":57,"title":["Expression of melatonin receptor MT<\/scp>1 in cells of human invasive ductal breast carcinoma"],"prefix":"10.1111","volume":"54","author":[{"given":"Karolina","family":"Jablonska","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology Wroclaw Medical University Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartosz","family":"Pula","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Wroclaw Medical University Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Zemla","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Wroclaw Medical University Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Owczarek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Pharmacology and Toxicology Wroclaw University of Environmental and Life Sciences Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Wojnar","sequence":"additional","affiliation":[{"name":"Department of Pathomorphology Lower Silesian Centre of Oncology Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janusz","family":"Rys","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology Centre of Oncology Maria Sklodowska\u2010Curie Memorial Institute, Cracow Branch Cracow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Ambicka","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology Centre of Oncology Maria Sklodowska\u2010Curie Memorial Institute, Cracow Branch Cracow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzena","family":"Podhorska\u2010Okolow","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Wroclaw Medical University Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maciej","family":"Ugorski","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Pharmacology and Toxicology Wroclaw University of Environmental and Life Sciences Wroclaw Poland"},{"name":"Laboratory of Glycobiology and Cellular Interactions Institute of Immunology and Experimental Therapy Polish Academy of Sciences Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Dziegiel","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Wroclaw Medical University Wroclaw Poland"},{"name":"Department of Physiotherapy Wroclaw University School of Physical Education Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)92591-6"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/274894a0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cdp.2006.03.002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/187221412800604581"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/156800908786733469"},{"key":"e_1_2_6_7_1","first-page":"425","article-title":"MT1 melatonin receptors and their role in the oncostatic action of melatonin [in Polish]","volume":"63","author":"Danielczyk K","year":"2009","journal-title":"Postepy Hig Med Dosw"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21401"},{"key":"e_1_2_6_9_1","first-page":"3403","article-title":"Effect of melatonin on mammary carcinogenesis in intact and pinealectomized rats in varying photoperiods","volume":"44","author":"Shah PN","year":"1984","journal-title":"Cancer Res"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2012.00973.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.2012.681045"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1995.tb00136.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00967.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-12-2-151"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2012.01.004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00856.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01923947"},{"key":"e_1_2_6_18_1","first-page":"57","article-title":"Melatonin: a potent, endogenous hydroxyl radical scavenger","volume":"1","author":"Tan DX","year":"1993","journal-title":"Endocr J"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2007_3264"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00916.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.13.7037"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2012.00995.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smrv.2008.07.007"},{"key":"e_1_2_6_24_1","first-page":"RA64","article-title":"Melatonin: adjuvant therapy of malignant tumors","volume":"14","author":"Dziegiel P","year":"2008","journal-title":"Med Sci Monit"},{"key":"e_1_2_6_25_1","first-page":"3887","article-title":"The expression of MT1 melatonin receptor and Ki\u201067 antigen in melanoma malignum","volume":"29","author":"Danielczyk K","year":"2009","journal-title":"Anticancer Res"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00940.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2004.00165.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2012.01000.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2012.01.003"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.184"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986710794183015"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1309\/1T4V-CT1G-UBJP-3EHP"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2008.04.001"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.13.12.0390"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2009.00684.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-011-9222-4"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/106689690201000409"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605750"},{"key":"e_1_2_6_39_1","first-page":"138","article-title":"Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER\u2010ICA) in breast cancer tissue","volume":"8","author":"Remmele W","year":"1987","journal-title":"Pathologe"},{"key":"e_1_2_6_40_1","first-page":"4945","article-title":"Comparison of metallothionein (MT) and Ki\u201067 antigen expression in benign and malignant thyroid tumours","volume":"30","author":"Krolicka A","year":"2010","journal-title":"Anticancer Res"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdp322"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012281221647"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7079745"},{"key":"e_1_2_6_45_1","first-page":"433","article-title":"Effects of melatonin on cancer: studies on MCF\u20107 human breast cancer cells in culture","volume":"21","author":"Blask DE","year":"1986","journal-title":"J Neural Transm"},{"key":"e_1_2_6_46_1","first-page":"6121","article-title":"Effects of the pineal hormone melatonin on the proliferation and morphological characteristics of human breast cancer cells (MCF\u20107) in culture","volume":"48","author":"Hill SM","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_6_47_1","first-page":"4383","article-title":"Influence of melatonin on invasive and metastatic properties of MCF\u20107 human breast cancer cells","volume":"58","author":"Cos S","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2010.12.004"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2009.00679.x"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-008-0220-1"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(90)90454-S"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-3045"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1006\/frne.1999.0194"},{"key":"e_1_2_6_54_1","first-page":"947","article-title":"Effects of MT1 melatonin receptor overexpression on the aromatase\u2010suppressive effect of melatonin in MCF\u20107 human breast cancer cells","volume":"17","author":"Gonz\u00e1lez A","year":"2007","journal-title":"Oncol Rep"},{"key":"e_1_2_6_55_1","first-page":"173","article-title":"The role of melatonin in breast, ovarian and endometrial cancer","volume":"38","author":"Jablonska K","year":"2010","journal-title":"Post Biol Kom"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1995.164"},{"key":"e_1_2_6_57_1","first-page":"302","article-title":"A phase II study of anastrozole plus pineal anticancer hormone melatonin in the metastatic breast cancer women with poor clinical status","volume":"7","author":"Lissoni P","year":"2009","journal-title":"Cancer Ther"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2004.00207.x"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1462399409000982"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00029-1"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr2794"},{"key":"e_1_2_6_62_1","first-page":"127","volume-title":"Immunohistochemistry and In Situ Hybridization of Human Carcinomas","author":"Dziegiel P","year":"2006"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-005-7755-9"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1159\/000226857"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2005.00258.x"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-4502"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1945"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-4194"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-010-0958-0"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.3109\/00207454.2010.492921"}],"container-title":["Journal of Pineal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpi.12032","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpi.12032","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.12032","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T04:56:01Z","timestamp":1698641761000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpi.12032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,17]]},"references-count":69,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1111\/jpi.12032"],"URL":"https:\/\/doi.org\/10.1111\/jpi.12032","archive":["Portico"],"relation":{},"ISSN":["0742-3098","1600-079X"],"issn-type":[{"value":"0742-3098","type":"print"},{"value":"1600-079X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,17]]}}}