{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T10:35:52Z","timestamp":1782815752486,"version":"3.54.5"},"reference-count":75,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,3,30]],"date-time":"2015-03-30T00:00:00Z","timestamp":1427673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003074","name":"ANPCyT","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"crossref"}]},{"name":"SECTyP-UNCuyo","award":["06\/J394"],"award-info":[{"award-number":["06\/J394"]}]},{"name":"FCM-UNCuyo","award":["138\/11CD"],"award-info":[{"award-number":["138\/11CD"]}]},{"name":"PICT-CONICET","award":["2006-451"],"award-info":[{"award-number":["2006-451"]}]},{"name":"PICT-CONICET","award":["2007-682"],"award-info":[{"award-number":["2007-682"]}]},{"name":"PICT-CONICET","award":["2012-174"],"award-info":[{"award-number":["2012-174"]}]},{"name":"PIP-CONICET","award":["112-201101-00247"],"award-info":[{"award-number":["112-201101-00247"]}]},{"name":"PIP-CONICET","award":["114-200901-00354"],"award-info":[{"award-number":["114-200901-00354"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Circadian rhythms govern many aspects of mammalian physiology. The daily pattern of melatonin synthesis and secretion is one of the classic examples of circadian oscillations. It is mediated by a class of neuroendocrine cells known as pinealocytes which are not yet fully defined. An established method to evaluate functional and cytological characters is through the expression of lineage\u2010specific transcriptional regulators. NeuroD1 is a basic helix\u2010loop\u2010helix transcription factor involved in the specification and maintenance of both endocrine and neuronal phenotypes. We have previously described developmental and adult regulation of NeuroD1 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>in the rodent pineal gland. However, the transcript levels were not influenced by the elimination of sympathetic input, suggesting that any rhythmicity of NeuroD1 might be found downstream of transcription. Here, we describe NeuroD1 protein expression and cellular localization in the rat pineal gland during development and the daily cycle. In embryonic and perinatal stages, protein expression follows the m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>pattern and is predominantly nuclear. Thereafter, NeuroD1 is mostly found in pinealocyte nuclei in the early part of the night and in cytoplasm during the day, a rhythm maintained into adulthood. Additionally, nocturnal nuclear NeuroD1 levels are reduced after sympathetic disruption, an effect mimicked by the in vivo administration of<jats:italic>\u03b1<\/jats:italic>\u2010 and<jats:italic>\u03b2<\/jats:italic>\u2010adrenoceptor blockers. NeuroD1 phosphorylation at two sites, Ser<jats:sup>274<\/jats:sup>and Ser<jats:sup>336<\/jats:sup>, associates with nuclear localization in pinealocytes. These data suggest that NeuroD1 influences pineal phenotype both during development and adulthood, in an autonomic and phosphorylation\u2010dependent manner.<\/jats:p>","DOI":"10.1111\/jpi.12228","type":"journal-article","created":{"date-parts":[[2015,3,6]],"date-time":"2015-03-06T15:36:44Z","timestamp":1425656204000},"page":"439-451","source":"Crossref","is-referenced-by-count":17,"title":["Expression and cellular localization of the transcription factor<scp>N<\/scp>euro<scp>D<\/scp>1 in the developing and adult rat pineal gland"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6701-1535","authenticated-orcid":false,"given":"Anal\u00eda E.","family":"Castro","sequence":"first","affiliation":[{"name":"Laboratory of Neurobiology: Chronobiology Section Institute of Histology and Embryology of Mendoza (IHEM\u2010CONICET) School of Medicine National University of Cuyo Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio G.","family":"Benitez","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology: Chronobiology Section Institute of Histology and Embryology of Mendoza (IHEM\u2010CONICET) School of Medicine National University of Cuyo Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz E.","family":"Farias Altamirano","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology: Chronobiology Section Institute of Histology and Embryology of Mendoza (IHEM\u2010CONICET) School of Medicine National University of Cuyo Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis E.","family":"Savastano","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology: Chronobiology Section Institute of Histology and Embryology of Mendoza (IHEM\u2010CONICET) School of Medicine National University of Cuyo Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean I.","family":"Patterson","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology: Traumatic and Toxic Lesions of the Nervous System Section Institute of Histology and Embryology of Mendoza (IHEM\u2010CONICET) School of Medicine National University of Cuyo Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estela M.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology: Chronobiology Section Institute of Histology and Embryology of Mendoza (IHEM\u2010CONICET) School of Medicine National University of Cuyo Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/07420520500545839"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04435.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-012-0906-y"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"3279","DOI":"10.1242\/dev.121.10.3279","article-title":"Forebrain and midbrain regions are deleted in Otx2\u2212\/\u2212 mutants due to a defective anterior neuroectoderm specification during gastrulation","volume":"121","author":"Acampora D","year":"1995","journal-title":"Development"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.21.2646"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00527-5"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10576"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1155"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.03773.x"},{"key":"e_1_2_8_11_1","first-page":"2074","article-title":"PAX6 aniridia and interhemispheric brain anomalies","volume":"15","author":"Abouzeid H","year":"2009","journal-title":"Mol Vis"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1700\/s00429\u2010014\u20100740\u2010x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.4.1876"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/70591"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07405.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1232"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01209-07"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01129-0"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0953-816X.2000.01324.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000058547"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2003.10.019"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000327430"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/35039080"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203909200"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1081\/CBI-120022525"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1226339"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7754368"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.8.1009"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.3560"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.11.6673"},{"key":"e_1_2_8_31_1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S1016-8478(23)13113-X","article-title":"NeuroD: the predicted and the surprising","volume":"18","author":"Chae JH","year":"2004","journal-title":"Mol Cells"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1385\/MN:30:1:035"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4832-07.2008"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi701527r"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.1.23"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00452"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-02-00453.2003"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730404267340"},{"key":"e_1_2_8_39_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/0-387-32442-9_23","article-title":"A role for bHLH transcription factors in retinal degeneration and dysfunction","volume":"572","author":"Pennesi ME","year":"2006","journal-title":"Adv Exp Med Biol"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04605.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2012.07870.x"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2008.12.005"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0834"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2009.00738.x"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2010.07.007"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.09.083"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102056"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00841-9"},{"key":"e_1_2_8_49_1","first-page":"137","article-title":"Time of origin of the rat pineal gland cells. A bromodeoxyuridine immunohistochemical study","volume":"19","author":"Calvo JL","year":"2004","journal-title":"Histol Histopathol"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004120050256"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2007.03.001"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808394200"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1998.00309.x"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-1180"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03318-5"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301198200"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.12.004"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730408328438"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2007.06.001"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2555-10.2011"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.visres.2010.10.016"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.085886"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.147215"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20031021"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0882"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05765.x"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.13.1647"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.2.865"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066487"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701762200"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10-10-0809"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.2.2"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R600036200"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.03.022"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04855.x"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0007-1331.2001.00738.x"}],"container-title":["Journal of Pineal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpi.12228","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpi.12228","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.12228","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T17:30:43Z","timestamp":1717781443000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpi.12228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,30]]},"references-count":75,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1111\/jpi.12228"],"URL":"https:\/\/doi.org\/10.1111\/jpi.12228","archive":["Portico"],"relation":{},"ISSN":["0742-3098","1600-079X"],"issn-type":[{"value":"0742-3098","type":"print"},{"value":"1600-079X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,30]]}}}