{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:15:57Z","timestamp":1787015757863,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,4]],"date-time":"2015-04-04T00:00:00Z","timestamp":1428105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo, FAPESP","award":["2009\/02319-0"],"award-info":[{"award-number":["2009\/02319-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2015,5]]},"abstract":"Abstract<\/jats:title>\n \n This study investigated the maturational stage (immature and mature ovaries) differences of m\n RNA<\/jats:styled-content>\n expression of melatonin\u2010forming enzymes (\n Aanat<\/jats:italic>\n and\n Asmt<\/jats:italic>\n ), melatonin membrane receptors (\n Mt1<\/jats:italic>\n and\n Mt2<\/jats:italic>\n ) and putative nuclear (\n Ror\u03b1<\/jats:italic>\n ) receptors, and clock genes (\n Clock<\/jats:italic>\n ,\n Bmal1<\/jats:italic>\n ,\n Per1<\/jats:italic>\n ,\n Per2<\/jats:italic>\n ,\n Cry1<\/jats:italic>\n ,\n Cry2<\/jats:italic>\n ) in cumulus\u2013oocyte complexes (\n COC<\/jats:styled-content>\n ) from weaning Wistar rats. We also examined the effects of pinealectomy and of melatonin pharmacological replacement on the daily expression of these genes in\n COC<\/jats:styled-content>\n . q\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n analysis revealed that in oocytes, the m\n RNA<\/jats:styled-content>\n expression of\n Asmt<\/jats:italic>\n ,\n Mt2<\/jats:italic>\n ,\n Clock<\/jats:italic>\n ,\n Bmal1<\/jats:italic>\n ,\n Per2<\/jats:italic>\n , and\n Cry1<\/jats:italic>\n were higher (\n P<\/jats:italic>\n \u00a0<\u00a00.05) in immature ovaries than in the mature ones. In cumulus cells, the same pattern of m\n RNA<\/jats:styled-content>\n expression for\n Asmt<\/jats:italic>\n ,\n Aanat<\/jats:italic>\n ,\n Ror\u03b1<\/jats:italic>\n ,\n Clock<\/jats:italic>\n ,\n Per1<\/jats:italic>\n ,\n Cry1<\/jats:italic>\n , and\n Cry2<\/jats:italic>\n genes was observed. In oocytes, pinealectomy altered the daily m\n RNA<\/jats:styled-content>\n expression profiles of\n Asmt<\/jats:italic>\n ,\n Mt1<\/jats:italic>\n ,\n Mt2<\/jats:italic>\n ,\n Clock<\/jats:italic>\n ,\n Per1<\/jats:italic>\n ,\n Cry1<\/jats:italic>\n , and\n Cry2<\/jats:italic>\n genes. In cumulus cells, removal of the pineal altered the\n mRNA<\/jats:styled-content>\n expression profiles of\n Mt1<\/jats:italic>\n ,\n Mt2<\/jats:italic>\n ,\n Ror\u03b1<\/jats:italic>\n ,\n Aanat<\/jats:italic>\n ,\n Asmt<\/jats:italic>\n ,\n Clock<\/jats:italic>\n ,\n Bmal1<\/jats:italic>\n ,\n Per2<\/jats:italic>\n ,\n Cry1<\/jats:italic>\n , and\n Cry2<\/jats:italic>\n genes. Melatonin treatment partially or completely re\u2010established the daily m\n RNA<\/jats:styled-content>\n expression profiles of most genes studied. The m\n RNA<\/jats:styled-content>\n expression of melatonin\u2010related genes and clock genes in rat\n COC<\/jats:styled-content>\n varies with the maturational stage of the meiotic cellular cycle in addition to the hour of the day. This suggests that melatonin might act differentially in accordance with the maturational stage of cumulus\/oocyte complex. In addition, it seems that circulating pineal melatonin is very important in the design of the daily profile of m\n RNA<\/jats:styled-content>\n expression of\n COC<\/jats:styled-content>\n clock genes and genes related to melatonin synthesis and action.\n <\/jats:p>","DOI":"10.1111\/jpi.12234","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T12:23:52Z","timestamp":1427199832000},"page":"490-499","source":"Crossref","is-referenced-by-count":60,"title":["Daily differential expression of melatonin\u2010related genes and clock genes in rat cumulus\u2013oocyte complex: changes after pinealectomy"],"prefix":"10.1111","volume":"58","author":[{"given":"L. A.","family":"Coelho","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine Faculty of Animal Science and Food Engineering (FZEA) University of S\u00e3o Paulo (USP) Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Peres","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics Institute of Biomedical Sciences (ICB) University of S\u00e3o Paulo (USP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4492-4822","authenticated-orcid":false,"given":"F. G.","family":"Amaral","sequence":"additional","affiliation":[{"name":"Department of Physiology Federal University of S\u00e3o Paulo UNIFESP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Reiter","sequence":"additional","affiliation":[{"name":"Department of Cellular and Structural Biology University of Texas Health Science Center San Antonio TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Cipolla\u2010Neto","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics Institute of Biomedical Sciences (ICB) University of S\u00e3o Paulo (USP) S\u00e3o Paulo 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