{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:17:48Z","timestamp":1790201868620,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,6,11]],"date-time":"2016-06-11T00:00:00Z","timestamp":1465603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology of Taiwan","doi-asserted-by":"crossref","award":["NSC-99-2320-B-006-019-MY3"],"award-info":[{"award-number":["NSC-99-2320-B-006-019-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology of Taiwan","doi-asserted-by":"crossref","award":["NSC-102-2320-B-006-012-MY3"],"award-info":[{"award-number":["NSC-102-2320-B-006-012-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2016,9]]},"abstract":"Abstract<\/jats:title>\n \n Mitochondrial dysfunction and oxidative stress are involved in the pathogenesis of\n P<\/jats:styled-content>\n arkinson's disease (\n PD<\/jats:styled-content>\n ). Mitochondrial morphology is dynamic and precisely regulated by the mitochondrial fission and fusion machinery. Aberrant mitochondrial fragmentation controlled by the mitochondrial fission protein, dynamin\u2010related protein 1 (\n D<\/jats:styled-content>\n rp1), may result in cell death. Our previous results showed that melatonin protected neurons by inhibiting oxidative stress in a 1\u2010methyl\u20104\u2010phenylpyridinium (\n MPP<\/jats:styled-content>\n +<\/jats:sup>\n )\u2010induced\n PD<\/jats:styled-content>\n model. However, the effect of melatonin on mitochondrial dynamics remains uncharacterized. Herein, we investigated the effect of melatonin and the role of\n D<\/jats:styled-content>\n rp1 on\n MPP<\/jats:styled-content>\n +<\/jats:sup>\n \u2010induced mitochondrial fission in rat primary cortical neurons. We found that\n MPP<\/jats:styled-content>\n +<\/jats:sup>\n induced a rapid increase in the ratio of\n GSSG<\/jats:styled-content>\n :total glutathione (a marker of oxidative stress) and mitochondrial fragmentation,\n D<\/jats:styled-content>\n rp1 upregulation within 4\u00a0hours, and finally resulted in neuron loss 48\u00a0hours after the treatment. Neurons overexpressing wild\u2010type\n D<\/jats:styled-content>\n rp1 promoted mitochondrial and nuclear fragmentation; however, neurons overexpressing dominant\u2010negative\n D<\/jats:styled-content>\n rp1\n K38A<\/jats:sup>\n or cotreated with melatonin exhibited significantly reduced\n MPP<\/jats:styled-content>\n +<\/jats:sup>\n \u2010induced mitochondrial fragmentation and neuron death. Moreover, melatonin cotreatment prevented an\n MPP<\/jats:styled-content>\n +<\/jats:sup>\n \u2010induced high ratio of\n GSSG<\/jats:styled-content>\n and mitochondrial\n D<\/jats:styled-content>\n rp1 upregulation. The prevention of mitochondrial fission by melatonin was not found in neurons transfected with wild\u2010type\n D<\/jats:styled-content>\n rp1. These results provide a new insight that the neuroprotective effect of melatonin against\n MPP<\/jats:styled-content>\n +<\/jats:sup>\n toxicity is mediated by inhibiting the oxidative stress and\n D<\/jats:styled-content>\n rp1\u2010mediated mitochondrial fragmentation.\n <\/jats:p>","DOI":"10.1111\/jpi.12343","type":"journal-article","created":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T14:57:10Z","timestamp":1462805830000},"page":"230-240","source":"Crossref","is-referenced-by-count":75,"title":["Melatonin prevents the dynamin\u2010related protein 1\u2010dependent mitochondrial fission and oxidative insult in the cortical neurons after 1\u2010methyl\u20104\u2010phenylpyridinium treatment"],"prefix":"10.1111","volume":"61","author":[{"given":"Jih\u2010Ing","family":"Chuang","sequence":"first","affiliation":[{"name":"Department of Physiology College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I\u2010Ling","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Physiology College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Yun","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Physiology College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiu\u2010Ying","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Physiology College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Chun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physiology College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyh Wei","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Parasitology College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.22.010305.104638"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2012.18"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds311"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2009.03.006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.213538"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.144238"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2012.07734.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808515200"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2011.00721.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.03.009"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05292"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-079X.2002.01871.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410360306"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.05.001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.08.002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2012.128"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1171091"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601253"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703812200"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12162"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12267"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12254"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1600-079X.2003.00107.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00861.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2009.00700.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12078"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.06.026"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2009.06061.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(85)13073-9"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.02.064"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00607.2004"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402087"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114439"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0930-14.2014"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds352"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005546"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfs218"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn104"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-013-9505-0"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-079X.2000.280407.x"}],"container-title":["Journal 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