{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:57:59Z","timestamp":1790121479645,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T00:00:00Z","timestamp":1497916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T00:00:00Z","timestamp":1497916800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81501920"],"award-info":[{"award-number":["81501920"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573434"],"award-info":[{"award-number":["81573434"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573425"],"award-info":[{"award-number":["81573425"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["LC2015027"],"award-info":[{"award-number":["LC2015027"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["H2015056"],"award-info":[{"award-number":["H2015056"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003999","name":"Science Fund for Creative Research Groups","doi-asserted-by":"publisher","award":["81421063"],"award-info":[{"award-number":["81421063"]}],"id":[{"id":"10.13039\/501100003999","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","award":["20132307120023"],"award-info":[{"award-number":["20132307120023"]}],"id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2017,10]]},"abstract":"Abstract<\/jats:title>\n \n Bone marrow mesenchymal stem cells (\n BMSC<\/jats:styled-content>\n s) are an expandable population of stem cells which can differentiate into osteoblasts, chondrocytes and adipocytes. Dysfunction of\n BMSC<\/jats:styled-content>\n s in response to pathological stimuli contributes to bone diseases. Melatonin, a hormone secreted from pineal gland, has been proved to be an important mediator in bone formation and mineralization. The aim of this study was to investigate whether melatonin protected against iron overload\u2010induced dysfunction of\n BMSC<\/jats:styled-content>\n s and its underlying mechanisms. Here, we found that iron overload induced by ferric ammonium citrate (\n FAC<\/jats:styled-content>\n ) caused irregularly morphological changes and markedly reduced the viability in\n BMSC<\/jats:styled-content>\n s. Consistently, osteogenic differentiation of\n BMSC<\/jats:styled-content>\n s was significantly inhibited by iron overload, but melatonin treatment rescued osteogenic differentiation of\n BMSC<\/jats:styled-content>\n s. Furthermore, exposure to\n FAC<\/jats:styled-content>\n led to the senescence in\n BMSC<\/jats:styled-content>\n s, which was attenuated by melatonin as well. Meanwhile, melatonin was able to counter the reduction in cell proliferation by iron overload in\n BMSC<\/jats:styled-content>\n s. In addition, protective effects of melatonin on iron overload\u2010induced dysfunction of\n BMSC<\/jats:styled-content>\n s were abolished by its inhibitor luzindole. Also, melatonin protected\n BMSC<\/jats:styled-content>\n s against iron overload\u2010induced\n ROS<\/jats:styled-content>\n accumulation and membrane potential depolarization. Further study uncovered that melatonin inhibited the upregulation of p53,\n ERK<\/jats:styled-content>\n and p38 protein expressions in\n BMSC<\/jats:styled-content>\n s with iron overload. Collectively, melatonin plays a protective role in iron overload\u2010induced osteogenic differentiation dysfunction and senescence through blocking\n ROS<\/jats:styled-content>\n accumulation and p53\/\n ERK<\/jats:styled-content>\n \/p38 activation.\n <\/jats:p>","DOI":"10.1111\/jpi.12422","type":"journal-article","created":{"date-parts":[[2017,5,13]],"date-time":"2017-05-13T10:46:39Z","timestamp":1494672399000},"source":"Crossref","is-referenced-by-count":120,"title":["Melatonin protects bone marrow mesenchymal stem cells against iron overload\u2010induced aberrant differentiation and senescence"],"prefix":"10.1111","volume":"63","author":[{"given":"Fan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pharmacy the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University) Harbin China"},{"name":"Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical Sciences Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University) Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gege","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University) Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University) Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University) Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University) Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University) Harbin China"},{"name":"Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical Sciences Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Second Affiliated Hospital of Harbin Medical University (Institute of Clinical Pharmacy, the Heilongjiang Key Laboratory of Drug Research, Harbin Medical University) Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elina","family":"Idiiatullina","sequence":"additional","affiliation":[{"name":"Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical Sciences Harbin China"},{"name":"Central Laboratory of Scientific Research Bashkir State Medical University Ufa Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timur","family":"Bikkuzin","sequence":"additional","affiliation":[{"name":"Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical Sciences Harbin China"},{"name":"Central Laboratory of Scientific Research Bashkir State Medical University Ufa Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Pavlov","sequence":"additional","affiliation":[{"name":"Central Laboratory of Scientific Research Bashkir State Medical University Ufa 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