{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:26:30Z","timestamp":1787495190369,"version":"build-2736575974"},"reference-count":90,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T00:00:00Z","timestamp":1515456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 HL066367"],"award-info":[{"award-number":["RO1 HL066367"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006710","name":"Wayne State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2018,4]]},"abstract":"Abstract<\/jats:title>\n \n Hypochlorous acid (\n HOC<\/jats:styled-content>\n l) is a potent cytotoxic oxidant generated by the enzyme myeloperoxidase (\n MPO<\/jats:styled-content>\n ) in the presence of hydrogen peroxide (H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n ) and chloride (Cl\n \u2212<\/jats:sup>\n ). Elevated levels of\n HOC<\/jats:styled-content>\n l play an important role in various pathological conditions through oxidative modification of several biomolecules. Recently, we have highlighted the ability of\n HOC<\/jats:styled-content>\n l to mediate the destruction of the metal\u2010ion derivatives of tetrapyrrole macrocyclic rings such as hemoproteins and vitamin B\n 12<\/jats:sub>\n (\n VB<\/jats:styled-content>\n 12<\/jats:sub>\n ) derivatives. Destruction of cyanocobalamin, a common pharmacological form of\n VB<\/jats:styled-content>\n 12<\/jats:sub>\n mediated by\n HOC<\/jats:styled-content>\n l, results in the generation of toxic molecular products such as chlorinated derivatives, corrin ring cleavage products, the toxic blood agents cyanide (\n CN<\/jats:styled-content>\n \u2212<\/jats:sup>\n ) and cyanogen chloride (\n CNC<\/jats:styled-content>\n l), and redox\u2010active free cobalt. Here, we show that melatonin prevents\n HOC<\/jats:styled-content>\n l\u2010mediated cyanocobalamin destruction, using a combination of\n UV<\/jats:styled-content>\n \u2010Vis spectrophotometry, high\u2010performance liquid chromatography analysis, and colorimetric\n CNC<\/jats:styled-content>\n l assay. Identification of several melatonin oxidation products suggests that the protective role of melatonin against\n HOC<\/jats:styled-content>\n l\u2010mediated cyanocobalamin destruction and subsequent\n CNC<\/jats:styled-content>\n l generation is at the expense of melatonin oxidation. Collectively, this work highlights that, in addition to acting as an antioxidant and as a\n MPO<\/jats:styled-content>\n inhibitor, melatonin can also prevent\n VB<\/jats:styled-content>\n 12<\/jats:sub>\n deficiency in inflammatory conditions such as cardiovascular and neurodegenerative diseases, among many others.\n <\/jats:p>","DOI":"10.1111\/jpi.12463","type":"journal-article","created":{"date-parts":[[2017,12,16]],"date-time":"2017-12-16T17:44:23Z","timestamp":1513446263000},"source":"Crossref","is-referenced-by-count":27,"title":["Melatonin prevents hypochlorous acid\u2010mediated cyanocobalamin destruction and cyanogen chloride generation"],"prefix":"10.1111","volume":"64","author":[{"given":"Roohi","family":"Jeelani","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology C. S. Mott Center for Human Growth and Development Wayne State University School of Medicine Detroit MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhiman","family":"Maitra","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology C. S. Mott Center for Human Growth and Development Wayne State University School of Medicine Detroit MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charalampos","family":"Chatzicharalampous","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology C. S. Mott Center for Human Growth and Development Wayne State University School of Medicine Detroit MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed","family":"Najeemuddin","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology C. S. 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