{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T06:05:04Z","timestamp":1788501904001,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T00:00:00Z","timestamp":1540166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/QUI\/50006\/2013"],"award-info":[{"award-number":["UID\/QUI\/50006\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/70781\/2010"],"award-info":[{"award-number":["SFRH\/BPD\/70781\/2010"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","award":["POCI\u201001\u20100145\u2010FEDER\u2010007491"],"award-info":[{"award-number":["POCI\u201001\u20100145\u2010FEDER\u2010007491"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["Project UID\/Multi \/00709\/2013"],"award-info":[{"award-number":["Project UID\/Multi \/00709\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["POCI\u201001\u20100145\u2010FEDER\u2010007265"],"award-info":[{"award-number":["POCI\u201001\u20100145\u2010FEDER\u2010007265"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cerebrospinal fluid melatonin is released from the pineal gland, directly into the third ventricle, or produced de novo in the brain from extrapineal melatonin sources leading to a melatonin concentration gradient in the cerebrospinal fluid. Despite the interest on this topic, the brain areas capable of producing melatonin are not yet clear. Bearing this in mind, we hypothesized that the choroid plexus (CP) could be one of these melatonin sources. We analyzed and confirmed the presence of the four enzymes required for melatonin synthesis in rat CP and demonstrated that arylalkylamine <jats:italic>N<\/jats:italic>\u2010acetyltransferase shows a circadian expression in female and male rat CP. Specifically, this enzyme colocalizes with mitochondria in rat CP epithelial cells, an organelle known to be involved in melatonin function and synthesis. Then, we demonstrated that melatonin is synthesized by porcine CP explants, although without a circadian pattern. In conclusion, our data show that the CP is a local source of melatonin to the central nervous system, probably contributing to its high levels in the cerebrospinal fluid. We believe that in the CP, melatonin might be regulated by its endogenous clock machinery and by the hormonal background.<\/jats:p>","DOI":"10.1111\/jpi.12528","type":"journal-article","created":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T14:58:21Z","timestamp":1538060301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Choroid plexus is an additional source of melatonin in the brain"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5202-9858","authenticated-orcid":false,"given":"Telma","family":"Quintela","sequence":"first","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Silva","sequence":"additional","affiliation":[{"name":"LAQV\u2010REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Duarte","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Guedes","sequence":"additional","affiliation":[{"name":"CENSE, DCEA, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e9ssia","family":"Andrade","sequence":"additional","affiliation":[{"name":"LAQV\u2010REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via","family":"Freitas","sequence":"additional","affiliation":[{"name":"LAQV\u2010REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia Universidade Nova de Lisboa Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Talhada","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e2nia","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Tavares","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis A.","family":"Passarinha","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"},{"name":"UCIBIO\u2014Requimte, Department of Chemistry, Faculty of Sciences and Technology Universidade Nova de Lisboa Caparica Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Cipolla\u2010Neto","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology, Department of Physiology and Biophysics, Institute of Biomedical Sciences University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cec\u00edlia R. A.","family":"Santos","sequence":"additional","affiliation":[{"name":"CICS\u2010UBI\u2014Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01923947"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-3589(08)61167-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12336"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2005.00223.x"},{"key":"e_1_2_7_6_1","first-page":"307","article-title":"The melatonin rhythm\u2010generating enzyme: molecular regulation of serotonin N\u2010acetyltransferase in the pineal gland","volume":"52","author":"Klein DC","year":"1997","journal-title":"Recent Prog Horm Res"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2009.06457.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2609-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1705768114"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12026"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-079X.2001.300408.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.21.12281"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/5.5.402"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4492(80)90129-X"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2005.00268.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-0694fje"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01923948"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/157015910792246182"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2007.00466.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00931.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-014-0719-7"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2009.12.008"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623310394214"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2012.00034"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.23"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-014-0720-1"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03507-2"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.06.095"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2003-4-10-r61"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2006.00411.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-014-1579-2"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00954.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23020509"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17060939"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17122124"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00880.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1384-06.2006"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00856.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406871102"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.7.8770929"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/07420520500482066"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2015.11.018"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2006.00407.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-13-0118"},{"key":"e_1_2_7_46_1","first-page":"1","article-title":"The choroid plexus harbors a circadian oscillator modulated by estrogens","author":"Quintela T","year":"2017","journal-title":"Chronobiol Int"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2017.07.004"}],"container-title":["Journal of Pineal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpi.12528","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.12528","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T21:13:25Z","timestamp":1694898805000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpi.12528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,22]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/jpi.12528"],"URL":"https:\/\/doi.org\/10.1111\/jpi.12528","archive":["Portico"],"relation":{},"ISSN":["0742-3098","1600-079X"],"issn-type":[{"value":"0742-3098","type":"print"},{"value":"1600-079X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,22]]},"assertion":[{"value":"2018-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12528"}}