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Melatonin receptors are approved drug targets for insomnia, non\u201024\u2010hour sleep\u2010wake disorders, and major depressive disorders. In mammals, two melatonin receptors (MTRs) exist, MT<jats:sub>1<\/jats:sub> and MT<jats:sub>2<\/jats:sub>, belonging to the G protein\u2010coupled receptor (GPCR) superfamily. Similar to most other GPCRs, reliable antibodies recognizing melatonin receptors proved to be difficult to obtain. Here, we describe the development of the first monoclonal antibodies (mABs) for mouse MT<jats:sub>1<\/jats:sub> and MT<jats:sub>2<\/jats:sub>. Purified antibodies were extensively characterized for specific reactivity with mouse, rat, and human MT<jats:sub>1<\/jats:sub> and MT<jats:sub>2<\/jats:sub> by Western blot, immunoprecipitation, immunofluorescence, and proximity ligation assay. Several mABs were specific for either mouse MT<jats:sub>1<\/jats:sub> or MT<jats:sub>2<\/jats:sub>. None of the mABs cross\u2010reacted with rat MTRs, and some were able to react with human MTRs. The specificity of the selected mABs was validated by immunofluorescence microscopy in three established locations (retina, suprachiasmatic nuclei, pituitary gland) for MTR expression in mice using MTR\u2010KO mice as control. MT<jats:sub>2<\/jats:sub> expression was not detected in mouse insulinoma MIN6 cells or pancreatic beta\u2010cells. Collectively, we report the first monoclonal antibodies recognizing recombinant and native mouse melatonin receptors that will be valuable tools for future studies.<\/jats:p>","DOI":"10.1111\/jpi.12540","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T14:16:03Z","timestamp":1543241763000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Detection of recombinant and endogenous mouse melatonin receptors by monoclonal antibodies targeting the C\u2010terminal domain"],"prefix":"10.1111","volume":"66","author":[{"given":"Erika","family":"Cecon","sequence":"first","affiliation":[{"name":"Inserm U1016, Institut Cochin Paris France"},{"name":"CNRS UMR 8104 Paris France"},{"name":"Sorbonne Paris Cit\u00e9 Univ. 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Paris Descartes Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Friederich","sequence":"additional","affiliation":[{"name":"Molecular Diabetology, Faculty of Medicine University Hospital, TU Dresden Dresden Germany"},{"name":"Faculty of Medicine Paul Langerhans Institute Dresden (PLID) of the Helmholtz Center Munich at University Hospital Carl Gustav Carus, TU Dresden Dresden Germany"},{"name":"German Center for Diabetes Research (DZD) Munich Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Luc","family":"Guillaume","sequence":"additional","affiliation":[{"name":"Inserm U1016, Institut Cochin Paris France"},{"name":"CNRS UMR 8104 Paris France"},{"name":"Sorbonne Paris Cit\u00e9 Univ. Paris Descartes Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Keller","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics (MPI\u2010CBG) Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Knoch","sequence":"additional","affiliation":[{"name":"Molecular Diabetology, Faculty of Medicine University Hospital, TU Dresden Dresden Germany"},{"name":"Faculty of Medicine Paul Langerhans Institute Dresden (PLID) of the Helmholtz Center Munich at University Hospital Carl Gustav Carus, TU Dresden Dresden Germany"},{"name":"German Center for Diabetes Research (DZD) Munich Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raise","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Inserm U1016, Institut Cochin Paris France"},{"name":"CNRS UMR 8104 Paris France"},{"name":"Sorbonne Paris Cit\u00e9 Univ. 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