{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:15:29Z","timestamp":1787015729623,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T00:00:00Z","timestamp":1553040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002808","name":"Carlsbergfondet","doi-asserted-by":"publisher","award":["CF15-0515"],"award-info":[{"award-number":["CF15-0515"]}],"id":[{"id":"10.13039\/501100002808","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002808","name":"Carlsbergfondet","doi-asserted-by":"publisher","award":["CF17-0070"],"award-info":[{"award-number":["CF17-0070"]}],"id":[{"id":"10.13039\/501100002808","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF15OC0015988"],"award-info":[{"award-number":["NNF15OC0015988"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF17OC0026938"],"award-info":[{"award-number":["NNF17OC0026938"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011958","name":"Danmarks Frie Forskningsfond","doi-asserted-by":"publisher","award":["8020-00037B"],"award-info":[{"award-number":["8020-00037B"]}],"id":[{"id":"10.13039\/501100011958","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Homeobox genes encode transcription factors that regulate developmental processes; however, in the pineal gland, a neuroendocrine organ responsible for nocturnal melatonin synthesis, expression of the homeobox genes\n                    <jats:italic>Otx2<\/jats:italic>\n                    (orthodenticle homeobox 2) and\n                    <jats:italic>Crx<\/jats:italic>\n                    (cone\u2010rod homeobox) persists postnatally. We here show that OTX2 and CRX are exclusively present in melatonin\u2010producing pinealocytes of the rat pineal gland. To understand the roles of\n                    <jats:italic>Otx2<\/jats:italic>\n                    and\n                    <jats:italic>Crx<\/jats:italic>\n                    in the mature pineal gland, we used siRNA technology in cultured rat pinealocytes with the nocturnal situation mimicked by adding norepinephrine to the culture media. siRNA\u2010induced knockdown of\n                    <jats:italic>Otx2<\/jats:italic>\n                    was found to reduce expression levels of the enzymes involved in melatonin synthesis at both transcript and protein levels. Similar results were obtained when knocking down\n                    <jats:italic>Crx<\/jats:italic>\n                    . Knocking down\n                    <jats:italic>Otx2<\/jats:italic>\n                    and\n                    <jats:italic>Crx<\/jats:italic>\n                    simultaneously produced an even larger reduction in both transcript and protein levels of the melatonin\u2010producing enzymes and also reduced the levels of melatonin released to the culture media. These results suggest that\n                    <jats:italic>Otx2<\/jats:italic>\n                    and\n                    <jats:italic>Crx<\/jats:italic>\n                    , both alone and in combination, act to control pineal melatonin synthesis.\n                  <\/jats:p>","DOI":"10.1111\/jpi.12567","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T01:51:37Z","timestamp":1551145897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Homeobox genes in melatonin\u2010producing pinealocytes:\n                    <i>Otx2<\/i>\n                    and\n                    <i>Crx<\/i>\n                    act to promote hormone synthesis in the mature rat pineal gland"],"prefix":"10.1111","volume":"66","author":[{"given":"Kristian","family":"Rohde","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Hertz","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4047-6324","authenticated-orcid":false,"given":"Martin F.","family":"Rath","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R600036200"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2015-1888"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2009.07.010"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-002-0580-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.7.8770929"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/378783a0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5355.1358"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-012-0906-y"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80394-3"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.03773.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0882"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07385.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-014-0740-x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80439-0"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80440-7"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.02.016"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/70591"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.4.1876"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1232"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00555.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/358687a0"},{"issue":"10","key":"e_1_2_9_23_1","doi-asserted-by":"crossref","first-page":"3279","DOI":"10.1242\/dev.121.10.3279","article-title":"Forebrain and midbrain regions are deleted in Otx2\u2010\/\u2010 mutants due to a defective anterior neuroectoderm specification during gastrulation","volume":"121","author":"Acampora D","year":"1995","journal-title":"Development"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.1.243"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.21.2646"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1155"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(99)00142-9"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0205883"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808394200"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)02102-2"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2008.00627.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.62.2.544"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1177\/0748730404267340"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01209-07"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07405.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00574-8"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.14120"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12481"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.03.021"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412935200"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng793"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2005.12.011"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01959.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.169.3950.1093"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2005.00223.x"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2007.12.011"}],"container-title":["Journal of Pineal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpi.12567","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.12567","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpi.12567","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.12567","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T12:50:55Z","timestamp":1694350255000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpi.12567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,20]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1111\/jpi.12567"],"URL":"https:\/\/doi.org\/10.1111\/jpi.12567","archive":["Portico"],"relation":{},"ISSN":["0742-3098","1600-079X"],"issn-type":[{"value":"0742-3098","type":"print"},{"value":"1600-079X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,20]]},"assertion":[{"value":"2019-02-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12567"}}