{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:46:22Z","timestamp":1787168782888,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T00:00:00Z","timestamp":1566432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2017R1A6A1A03015642"],"award-info":[{"award-number":["NRF-2017R1A6A1A03015642"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2019R1I1A2A01041413"],"award-info":[{"award-number":["NRF-2019R1I1A2A01041413"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mammalian oocytes remain arrested at the first prophase of meiosis in ovarian follicles for an extended period. During this protracted arrest, oocytes are remarkably susceptible to the accumulation of DNA damage. Melatonin (N\u2010acetyl\u20105\u2010methoxytryptamine), a hormone secreted by the pineal gland, has diverse effects on various physiological processes. However, the effect of melatonin on DNA damage response in mammalian oocytes has not been explored. Here, we showed that melatonin protected mouse oocytes from DNA damage induced by double\u2010strand breaks (DSBs) during prophase arrest and subsequently improved oocyte quality. We found that DNA damage during prophase arrest impaired subsequent meiotic maturation and deteriorated oocyte quality, increasing chromosome fragmentation, spindle abnormality, mitochondrial aggregation, and oxidative stress. However, melatonin treatment during DNA damage accumulation at prophase improved meiotic maturation and relieved the quality decline of oocytes. In addition, melatonin inhibited the accumulation of DNA damage during prophase arrest by reducing the \u03b3\u2010H2AX levels. Although activated ATM levels were decreased by melatonin treatment, the effect of melatonin on DNA damage response was not a direct consequence of ATM inhibition. Instead, melatonin enhanced DNA repair via nonhomologous end\u2010joining (NHEJ) pathway. Interestingly, these actions of melatonin on DNA damage response are receptor\u2010independent in mouse oocytes. Therefore, our results demonstrated that melatonin protects oocytes from DNA damage during prophase arrest by enhancing DNA repair via NHEJ and subsequently prevents the deterioration of oocyte quality during meiotic maturation.<\/jats:p>","DOI":"10.1111\/jpi.12603","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T18:18:35Z","timestamp":1564683515000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Melatonin protects mouse oocytes from DNA damage by enhancing nonhomologous end\u2010joining repair"],"prefix":"10.1111","volume":"67","author":[{"given":"Jiyeon","family":"Leem","sequence":"first","affiliation":[{"name":"Department of Integrative Biotechnology, College of Biotechnology and Bioengineering Sungkyunkwan University Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang\u2010Yu","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Integrative Biotechnology, College of Biotechnology and Bioengineering Sungkyunkwan University Suwon Korea"},{"name":"Biomedical Institute for Convergence Sungkyunkwan University Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Radiation Cancer Research Korea Institute of Radiological and Medical Sciences Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1947-5028","authenticated-orcid":false,"given":"Jeong Su","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Integrative Biotechnology, College of Biotechnology and Bioengineering Sungkyunkwan University Suwon Korea"},{"name":"Biomedical Institute for Convergence Sungkyunkwan University Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-416024-8.00007-6"},{"key":"e_1_2_8_3_1","first-page":"2137","article-title":"Double\u2010strand breaks: signaling pathways and repair mechanisms","volume":"61","author":"Karagiannis TC","year":"2004","journal-title":"Cell Mol Life Sci"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01368"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05337"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.08.017"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2013.00117"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.03.063"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.24311"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/85798"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep224"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.039073"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2010.12.004"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-154450"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/074873049701200606"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.2011.00916.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2003_3637"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-64-4-865"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-71-2-493"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1757-2215-5-5"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2011.589925"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23052"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101680"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27351"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12024"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-079x.2000.280107.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1998.tb00371.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-14-1"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12416"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9553"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9706"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100466200"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.18.6679"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.11.054"},{"key":"e_1_2_8_35_1","first-page":"E14","article-title":"The effect of oocyte quality on development","volume":"82","author":"Krisher RL","year":"2004","journal-title":"J Anim Sci"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.03.034"},{"key":"e_1_2_8_37_1","first-page":"27","article-title":"Expression levels of two DNA repair\u2010related genes under 8 Gy ionizing radiation and 100 mg\/kg melatonin delivery in rat peripheral blood","volume":"7","author":"Valizadeh M","year":"2017","journal-title":"J Biomed Phys Eng"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/074873001129002051"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0145-09.2009"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-10-103"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17060939"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2013.879856"}],"container-title":["Journal of Pineal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpi.12603","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.12603","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpi.12603","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.12603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T11:55:34Z","timestamp":1693569334000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpi.12603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,22]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1111\/jpi.12603"],"URL":"https:\/\/doi.org\/10.1111\/jpi.12603","archive":["Portico"],"relation":{},"ISSN":["0742-3098","1600-079X"],"issn-type":[{"value":"0742-3098","type":"print"},{"value":"1600-079X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,22]]},"assertion":[{"value":"2019-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12603"}}