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Herein, we investigated the effects of melatonin, a ubiquitous physiological mediator that is found throughout animals and plants. In mammals, the pineal gland secretes this indoleamine into the blood circulation to exert an extensive repertoire of biological activities. Our in vitro assessment indicates an oncostatic capacity of melatonin in time\u2010dependent manner (24, 48, 72\u00a0hours) in highly pigmented MNT\u20101 melanoma cells. The similar pattern of regulation regarding cell viability was observed in amelanotic Sk\u2010Mel\u201028 cells. Subsequently, MNT\u20101 cells were tested for the first time for evaluation of melanin\/melatonin interaction. Thus primary, electron paramagnetic resonance (EPR) spectroscopy demonstrated that melatonin reduced melanin content. Artificially induced disturbances of melanogenesis by selected inhibitors (N\u2010phenylthiourea or kojic acid) were slightly antagonized by melatonin. Additionally, analysis using transmission electron microscopy has shown that melatonin, particularly at higher dose of 10<jats:sup>\u22123<\/jats:sup>\u00a0mol\/L, triggered the appearance of premelanosomes (stage I\u2010II of melanosome) and MNT\u20101 cells synthesize de novo endogenous melatonin shown by LC\u2010MS. In conclusion, these studies show a melanogenic\u2010like function of melatonin suggesting it as an advantageous agent for treatment of pigmentary disorders.<\/jats:p>","DOI":"10.1111\/jpi.12610","type":"journal-article","created":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T19:04:17Z","timestamp":1568833457000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Melatonin exerts oncostatic capacity and decreases melanogenesis in human MNT\u20101 melanoma cells"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1311-263X","authenticated-orcid":false,"given":"Konrad","family":"Kleszczy\u0144ski","sequence":"first","affiliation":[{"name":"Department of Dermatology University of M\u00fcnster M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae\u2010Kang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Dermatology 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