{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T09:51:07Z","timestamp":1787737867274,"version":"build-2784847793"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T00:00:00Z","timestamp":1576627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1A2B6001173"],"award-info":[{"award-number":["2018R1A2B6001173"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Melatonin, a major hormone of the pineal gland, exerts many beneficial effects on mitochondria. Several studies have shown that melatonin can protect against toxin\u2010induced oocyte quality impairment during maturation. However, there is little information regarding the beneficial effects of melatonin on toxin\u2010exposed early embryos, and the mechanisms underlying such effects have not been determined. Rotenone, a chemical widely used in agriculture, induces mitochondrial toxicity, therefore, damaging the reproductive system, impairing oocyte maturation, ovulation, and fertilization. We investigated whether melatonin attenuated rotenone exposure\u2010induced impairment of embryo development by its mitochondrial protection effect. Activated oocytes were randomly assigned to four groups: the control, melatonin treatment, rotenone\u2010exposed, and \u201crotenone\u00a0+\u00a0melatonin\u201d groups. Treatment with melatonin abrogated rotenone\u2010induced impairment of embryo development, mitochondrial dysfunction, and ATP deficiency, and significantly decreased oxidative stress and apoptosis. Melatonin also increased SIRT1 and PGC\u20101\u03b1 expression, which promoted mitochondrial biogenesis. SIRT1 knockdown or pharmacological inhibition abolished melatonin's ability to revert rotenone\u2010induced impairment. Thus, melatonin rescued rotenone\u2010induced impairment of embryo development by reducing ROS production and promoting mitochondrial biogenesis. This study shows that melatonin rescues toxin\u2010induced impairment of early porcine embryo development by promoting mitochondrial biogenesis.<\/jats:p>","DOI":"10.1111\/jpi.12627","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T04:00:52Z","timestamp":1574827252000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":136,"title":["Melatonin enhances mitochondrial biogenesis and protects against rotenone\u2010induced mitochondrial deficiency in early porcine embryos"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1829-1718","authenticated-orcid":false,"given":"Ying\u2010Jie","family":"Niu","sequence":"first","affiliation":[{"name":"Department of Animal Science Chungbuk National University Cheongju South 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