{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:49:05Z","timestamp":1788994145379,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Diabetic retinopathy (DR) is characterized as a microvascular disease. Nonproliferative diabetic retinopathy (NPDR) presents with alterations in retinal blood flow and vascular permeability, thickening of the basement membrane, loss of pericytes, and formation of acellular capillaries. Endothelial\u2013mesenchymal transition (EndMT) of retinal microvessels may play a critical role in advancing NPDR. Melatonin, a hormone primarily secreted by the pineal gland, is a promising therapeutic for DR. This study explored the EndMT in retinal microvessels of NPDR and its related mechanisms. The effect of melatonin on the retina of diabetic rats was evaluated by electroretinogram (ERG) and histopathologic slide staining. Furthermore, the effect of melatonin on human retinal microvascular endothelial cells (HRMECs) was detected by EdU incorporation assay, scratch assay, transwell assay, and tube formation test. Techniques such as RNA\u2010sequencing, overexpression or knockdown of target genes, extraction of cytoplasmic and nuclear protein, co\u2010immunoprecipitation (co\u2010IP), and multiplex immunofluorescence facilitated the exploration of the mechanisms involved. Our findings reveal, for the first time, that melatonin attenuates diabetic retinopathy by regulating EndMT of retinal vascular endothelial cells via inhibiting the HDAC7\/FOXO1\/ZEB1 axis. Collectively, these results suggest that melatonin holds potential as a therapeutic strategy to reduce retinal vascular damage and protect vision in NPDR.<\/jats:p>","DOI":"10.1111\/jpi.13008","type":"journal-article","created":{"date-parts":[[2024,9,20]],"date-time":"2024-09-20T05:54:11Z","timestamp":1726811651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Melatonin Attenuates Diabetic Retinopathy by Regulating EndMT of Retinal Vascular Endothelial Cells via Inhibiting the HDAC7\/FOXO1\/ZEB1 Axis"],"prefix":"10.1111","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6009-347X","authenticated-orcid":false,"given":"Jiayi","family":"Ning","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"},{"name":"Xi'an Medical University Xi'an Shaanxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghong","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Tangdu Hospital Air Force Medical University Xi'an China"},{"name":"Department of Cardiothoracic Surgery Western Theater Command Air Force Hospital Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanyi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"},{"name":"Xi'an Medical University Xi'an Shaanxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Tangdu Hospital Air Force Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"},{"name":"Department of Thoracic Surgery Shaanxi Provincial People's Hospital Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingtong","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery The 71st Group Army Hospital of PLA\/The Affiliated Huaihai Hospital of Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingke","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"},{"name":"Xi'an Medical University Xi'an Shaanxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"},{"name":"Xi'an Medical University Xi'an Shaanxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengming","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sida","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7753-8736","authenticated-orcid":false,"given":"Yuanyong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Tangdu Hospital Air Force Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2419-9707","authenticated-orcid":false,"given":"Xiaolong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Tangdu Hospital Air Force Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2584-443X","authenticated-orcid":false,"given":"Jing","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tangdu Hospital Air Force Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,19]]},"reference":[{"issue":"5","key":"e_1_2_11_2_1","article-title":"Anti\u2010Vascular Endothelial Growth Factors in Combination With Vitrectomy for Complications of Proliferative Diabetic Retinopathy","volume":"5","author":"Dervenis P.","year":"2023","journal-title":"Cochrane Database of Systematic Reviews"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1055087"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.62.13.14"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021024"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.64.7.13"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-020-0439-4"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.567682"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.03167-2020"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1050540"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.642352"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-018-4797-6"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2023.04.007"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12813"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.831660"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40779-022-00412-0"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-022-02477-z"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12802"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.721869"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1131993"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11020226"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28044-x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39864-w"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2020.8169"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00747"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-018-0023-y"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2021.108585"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9811361"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-022-02266-9"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI131178"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04901-x"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01146-12"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.149377"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12885"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-021-00670-2"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040849"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skad286"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-2038"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard-2021-221269"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13671-8"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz816"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2020.10.006"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-021-00626-9"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23179839"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12473"}],"container-title":["Journal of Pineal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.13008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T13:16:53Z","timestamp":1727270213000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpi.13008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":44,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/jpi.13008"],"URL":"https:\/\/doi.org\/10.1111\/jpi.13008","archive":["Portico"],"relation":{},"ISSN":["0742-3098","1600-079X"],"issn-type":[{"value":"0742-3098","type":"print"},{"value":"1600-079X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2024-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13008"}}