{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T21:03:48Z","timestamp":1773263028312,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2024,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n The determination of melatonin levels in saliva represents one of the key methods for assessing the timing of the central circadian clock in humans, both in research and clinical settings. Melatonin levels in saliva are typically determined in a laboratory setting by RIA or enzyme\u2010linked immunosorbent assay and the emergence of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) presented a serious challenge to the routine, safe assessment of melatonin in saliva samples. However, SARS\u2010CoV\u20102 present in biological fluids can be inactivated by exposure to temperatures of at least 55\u201360\u00b0C for 30\u2009min and the aim of this study was to assess the validity of applying a pretreatment heating step to saliva samples being prepared for melatonin determination using the Novolytix Radioimmunoassay (RK\u2010DSM2). 40 archived saliva samples collected under a Dim Light Melatonin Onset sampling protocol were thawed and aliquoted into three identical groups\u2014Controls (no pretreatment), 56\u00b0C pre\u2010assay heat\u2010treatment (30\u2009min), and 70\u00b0C pre\u2010assay heat\u2010treatment (30\u2009min). Melatonin concentrations in samples that were heated to 56\u00b0C for 30\u2009min before assaying showed close agreement with the untreated controls, with the Pearson's correlation coefficient between the two sets of samples of 0.99 (\n p<\/jats:italic>\n \u2009<\u20090.0001) and the slope of the Deming regression analysis close to 1.0 (\n Y<\/jats:italic>\n \u2009=\u20091.04\n X<\/jats:italic>\n \u2009+\u20090.168). When saliva samples were pretreated to 70\u00b0C for 30\u2009min before assaying, the subsequent melatonin determinations were still strongly correlated with the untreated controls (Pearsons correlation coefficient\u2009=\u20090.97 (\n p<\/jats:italic>\n \u2009<\u20090.0001), however melatonin concentrations were consistently overestimated when compared to the untreated controls with Deming regression slope of\n Y<\/jats:italic>\n \u2009=\u20091.26\n X<\/jats:italic>\n \u2009+\u20090.241. These results indicate that a 56\u00b0C pretreatment step is suitable for inclusion in standard operating protocols for melatonin determinations using the Novolytix RIA, as a way of effectively minimizing the potential for accidental pathogen exposure while handling saliva samples.\n <\/jats:p>","DOI":"10.1111\/jpi.70009","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T02:29:44Z","timestamp":1732069784000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Heat Treatment of Saliva to Reduce Infectious Disease Contamination Does Not Impact the Analysis of Melatonin by Radioimmunoassay"],"prefix":"10.1111","volume":"76","author":[{"given":"Mark D.","family":"Salkeld","sequence":"first","affiliation":[{"name":"Adelaide Research Assay Facility, Faculty of Health and Medical Sciences University of Adelaide Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5864-3514","authenticated-orcid":false,"given":"David J.","family":"Kennaway","sequence":"additional","affiliation":[{"name":"Adelaide Research Assay Facility, Faculty of Health and Medical Sciences University of Adelaide Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2020.04.005"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c00784"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa149"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5679"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01296-8"},{"key":"e_1_2_9_7_1","unstructured":"\"WHO Director\u2010General's Opening 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