{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:38:26Z","timestamp":1792201106289,"version":"4.4.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32130098"],"award-info":[{"award-number":["32130098"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302740"],"award-info":[{"award-number":["32302740"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402844"],"award-info":[{"award-number":["32402844"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20220577"],"award-info":[{"award-number":["BK20220577"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20230581"],"award-info":[{"award-number":["BK20230581"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Spontaneous parturition in both humans and sows occurs predominantly at night. Melatonin is thought to facilitate nocturnal parturition in humans by synergistically enhancing myometrial contractions with oxytocin, but the precise underlying mechanisms remain unclear. In this study,\n                    <jats:italic>in vivo<\/jats:italic>\n                    analyses showed that melatonin levels in both serum and myometrium were significantly increased in laboring sows, accompanied by increased melatonin synthesized in the myometrium. Moreover, exogenous melatonin administration to pregnant sows potentially shifted parturition time. Functional studies demonstrated that melatonin supplementation significantly enhanced spontaneous myometrial contractility and sensitivity to oxytocin. Mechanistic investigations revealed that transcription factors NRF2 and MAFF were involved in the signaling pathway of melatonin, enhancing myometrial contractility. Silencing NRF2 or MAFF in melatonin\u2010treated myometrial cells reduced the expression of contraction\u2010associated proteins (CAPs) and attenuated collagen gel contraction. Dual\u2010luciferase reporter assays indicated that NRF2 and MAFF directly targeted the promoters of key contraction\u2010associated genes, including\n                    <jats:italic>PTGS2<\/jats:italic>\n                    and\n                    <jats:italic>OXTR<\/jats:italic>\n                    . Additionally, molecular analysis revealed that melatonin enhanced myometrial contraction by binding to the MT2 receptor. Furthermore, our results showed that PKC participated in melatonin\u2010induced myometrial contraction by regulating NRF2 and MAFF expression. Collectively, our data reveal, for the first time, that the MT2\u2010PKC\u2010NRF2\/MAFF axis mediates melatonin\u2010enhanced myometrial contraction in sows. These findings advance our understanding of parturition initiation in mammals and may provide a novel theoretical basis for developing therapeutic strategies for preterm or delayed\/prolonged labor.\n                  <\/jats:p>","DOI":"10.1111\/jpi.70135","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T10:06:46Z","timestamp":1773914806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Melatonin Enhances the Myometrial Contraction Through MT2\u2010PKC\u2010NRF2\/MAFF Signaling Pathway in Sows"],"prefix":"10.1111","volume":"78","author":[{"given":"Tongjuan","family":"Niu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yewen","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guobin","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinglin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety, The Ministry of Education of China Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangpeng","family":"Liao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kemian","family":"Gou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu China"},{"name":"Institute of Reproduction and Metabolism Yangzhou University Yangzhou Jiangsu China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201747458"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00714"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.75072"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioac174"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2009.02.044"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2020.02.005"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2023.04.011"},{"key":"e_1_2_10_9_1","first-page":"33","article-title":"Progesterone Action in the Myometrium and Decidua in Preterm Birth","volume":"4","author":"Blanks A. M.","year":"2012","journal-title":"Facts, Views & Vision in ObGyn"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqaf009"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1008301107"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1011261"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod67.1.240"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12552"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2025.1618784"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2014.06.020"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2008-1723"},{"key":"e_1_2_10_18_1","first-page":"168","article-title":"The Behaviour of Suckling Piglets","volume":"43","author":"Fericean M. L.","year":"2011","journal-title":"Research Journal of Agricultural Science"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1993.264.4.E534"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.50.37"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12481"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1352586"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-13-0405"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-020-03206-9"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2016.1151"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.134606"},{"key":"e_1_2_10_27_1","doi-asserted-by":"crossref","unstructured":"S.ArrowsmithHuman Myometrial Contractility Myometrial Contractility Assays. In: Werry E. L. Reekie T. A. Kassiou M. editors. Oxytocin: Methods and Protocols. New York NY: Springer US; 2022. p. 29\u201342.","DOI":"10.1007\/978-1-0716-1759-5_2"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0252438"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-024-05978-y"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02723005"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-018-1379-x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-2137"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00616"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.70024"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12566"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.623038"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12894"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD22194"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12216"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.120612"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12030688"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2025.102269"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-1274-9057"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10121867"},{"key":"e_1_2_10_45_1","first-page":"199","article-title":"Melatonin Inhibits Spontaneous and Oxytocin\u2010Induced Contractions of Rat Myometrium In Vitro","volume":"22","author":"Ayar A.","year":"2001","journal-title":"Neuro Endocrinology Letters"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202402949R"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0271081"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/13510002.2025.2507557"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/prp2.70132"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xgen.2025.100887"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2025.119985"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.045450"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1487"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6943"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2018.1474992"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1128"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gav023"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.107779"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719118802062"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.3109\/15419060109080741"}],"container-title":["Journal of Pineal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.70135","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpi.70135","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpi.70135","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T06:16:13Z","timestamp":1774592173000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpi.70135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":59,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/jpi.70135"],"URL":"https:\/\/doi.org\/10.1111\/jpi.70135","archive":["Portico"],"relation":{},"ISSN":["0742-3098","1600-079X"],"issn-type":[{"value":"0742-3098","type":"print"},{"value":"1600-079X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70135"}}