{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:07:34Z","timestamp":1791576454607,"version":"4.3.4"},"reference-count":64,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2024YFD1501001"],"award-info":[{"award-number":["2024YFD1501001"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011456","name":"State Key Laboratory of Soil and Sustainable Agriculture","doi-asserted-by":"publisher","award":["SKLSSA25K04"],"award-info":[{"award-number":["SKLSSA25K04"]}],"id":[{"id":"10.13039\/501100011456","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472059"],"award-info":[{"award-number":["32472059"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41401284"],"award-info":[{"award-number":["41401284"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Iron (Fe) is indispensable for plant growth and development. Notably, melatonin (MT) serves as a key regulator to improve plant tolerance under nutrient stress conditions. Currently, the exact mechanism regulating MT's involvement in the rice Fe\u2010deficiency response remains to be elucidated. This study reveals that Fe\u2010deficiency stress triggers a rapid increase in endogenous MT levels in rice roots and induces the expression of MT synthesis\u2010related genes, underscoring the involvement of MT in the rice Fe\u2010starvation response. Exogenously applied MT rescues the root growth inhibition triggered by Fe deficiency and efficiently mitigates leaf chlorosis in rice. Mechanistically, MT decreases Fe retention in the root cell wall and its hemicellulose fraction, thereby promoting the remobilization of stored Fe and increasing soluble Fe content. Additionally, MT selectively reshapes the transport networks by robustly upregulating genes associated with Strategy II Fe acquisition and internal Fe transport\u2014including\n                    <jats:italic>Ferric Reductase Defective 3\u2010like 1<\/jats:italic>\n                    (\n                    <jats:italic>OsFRDL1<\/jats:italic>\n                    ),\n                    <jats:italic>Iron\u2010related transcription factor 3<\/jats:italic>\n                    (\n                    <jats:italic>OsIRO3<\/jats:italic>\n                    ),\n                    <jats:italic>Nicotianamine Aminotransferase 1<\/jats:italic>\n                    (\n                    <jats:italic>OsNAAT1<\/jats:italic>\n                    ),\n                    <jats:italic>OsNAS1<\/jats:italic>\n                    ,\n                    <jats:italic>OsNAS2<\/jats:italic>\n                    ,\n                    <jats:italic>Yellow Stripe\u2010Like 2<\/jats:italic>\n                    (\n                    <jats:italic>OsYSL2<\/jats:italic>\n                    ), and\n                    <jats:italic>OsYSL15<\/jats:italic>\n                    \u2014to accelerate root\u2010to\u2010shoot Fe translocation. Notably, this enhanced internal Fe recycling partially improves the plant's Fe nutritional status, which is consistent with a systemic negative feedback response that partially attenuates the core Strategy I ferrous iron transporters (OsIRT1\/2) along with root\u2010surface ferric reductase (FCR) activity and proton secretion. Moreover, nitric oxide (NO) acts as a pivotal downstream mediator in MT\u2010induced mitigation of the rice Fe\u2010starvation response. In conclusion, MT alleviates Fe deficiency in rice by promoting the remobilization of hemicellulose\u2010bound Fe and enhancing root\u2010to\u2010shoot Fe translocation in an NO\u2010dependent manner. These processes are accompanied by partial attenuation of root\u2010surface FCR activity, proton secretion, and\n                    <jats:italic>OsIRT1<\/jats:italic>\n                    \/\n                    <jats:italic>2<\/jats:italic>\n                    expression, revealing a coordinated adjustment between internal Fe reutilization and external Fe\u2010acquisition responses.\n                  <\/jats:p>","DOI":"10.1111\/jpi.70176","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T07:55:48Z","timestamp":1786348548000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Melatonin\u2013Nitric Oxide Signaling Pathway Alleviates Iron Starvation by Mobilizing Apoplastic Iron Pools and Reshaping Iron Transport in Rice (\n                    <i>Oryza sativa<\/i>\n                    L.)"],"prefix":"10.1111","volume":"78","author":[{"given":"Jing","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science Chinese Academy of Sciences Nanjing China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science Chinese Academy of Sciences Nanjing China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng Tong","family":"Jing","sequence":"additional","affiliation":[{"name":"Yangzhou Branch of Jiangsu Provincial Hydrology and Water Resources Survey Bureau Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Gang","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Eco\u2010Agricultural Biotechnology Around Hongze Lake\/Collaborative Innovation Center of Regional Modern Agriculture &amp; Environmental Protection Huaiyin Normal University Huai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science Chinese Academy of Sciences Nanjing China"},{"name":"University of Chinese Academy of Sciences Beijing China"},{"name":"Changshu National Agro\u2010Ecosystem Observation and Research Station, Institute of Soil Science Chinese Academy of Sciences Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science Chinese Academy of Sciences Nanjing China"},{"name":"University of Chinese Academy of Sciences Beijing China"},{"name":"Yingtan National Field Agro\u2010Ecosystem Observation and Research Station, Institute of Soil Science Chinese Academy of Sciences Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren Fang","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science Chinese Academy of Sciences Nanjing China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3636-2631","authenticated-orcid":false,"given":"Lu","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science Chinese Academy of Sciences Nanjing China"},{"name":"University of Chinese Academy of Sciences Beijing 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