{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:49:14Z","timestamp":1792118954585,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2013,5,2]],"date-time":"2013-05-02T00:00:00Z","timestamp":1367452800000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Methane is the most important anthropogenic contribution to climate change after carbon dioxide and represents a loss of feed energy for the animal, mainly for herbivorous species. However, our knowledge about the ecology of Archaea, the microbial group responsible for methane synthesis in the gut, is very poor. Moreover, it is well known that hindgut fermentation differs from rumen fermentation. The composition of archaeal communities in fermentation compartments of goats and rabbits were investigated using DGGE to generate fingerprints of archaeal <jats:italic>16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content><\/jats:italic> gene. Ruminal contents and faeces from five Murciano\u2010Granadina goats and caecal contents of five commercial White New Zealand rabbits were compared. Diversity profile of methanogenic archaea was carried out by PCR\u2010DGGE. Quantification of methanogenic archaea and the abundance relative to bacteria was determined by real\u2010time PCR. Methanogenic archaeal species were relatively constant across species. Dendrogram from DGGE of the methanogen community showed one cluster for goat samples with two sub\u2010clusters by type of sample (ruminal and faeces). In a second cluster, samples from rabbit were grouped. No differences were found either in richness or Shannon index as diversity indexes. Although the primer sets used was developed to investigate rumen methanogenic archaeal community, primers specificity did not affect the assessment of rabbit methanogen community structure. Rumen content showed the highest number or methanogenic archaea (log<jats:sub>10<\/jats:sub> 9.36), followed by faeces (log<jats:sub>10<\/jats:sub> 8.52) and showing rabbit caecum the lower values (log<jats:sub>10<\/jats:sub> 5.52). DGGE profile showed that pre\u2010gastric and hindgut fermenters hold a very different methanogen community. Rabbits hold a microbial community of similar complexity than that in ruminants but less abundant, which agrees with the type of fermentation profile.<\/jats:p>","DOI":"10.1111\/jpn.12049","type":"journal-article","created":{"date-parts":[[2013,5,2]],"date-time":"2013-05-02T22:05:00Z","timestamp":1367532300000},"page":"80-88","source":"Crossref","is-referenced-by-count":11,"title":["Comparative study of fermentation and methanogen community structure in the digestive tract of goats and rabbits"],"prefix":"10.1111","volume":"97","author":[{"given":"L.","family":"Abecia","sequence":"first","affiliation":[{"name":"Estaci\u00f3n Experimental del Zaid\u00edn CSIC Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Fondevila","sequence":"additional","affiliation":[{"name":"IUCA Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos Universidad de Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Rodr\u00edguez\u2010Romero","sequence":"additional","affiliation":[{"name":"IUCA Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos Universidad de Zaragoza Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n Experimental del Zaid\u00edn CSIC Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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