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Conjugated linoleic acids (<jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content>), mainly the <jats:italic>trans<\/jats:italic>\u201010, <jats:italic>cis<\/jats:italic>\u201012 isomer, reduce milk fat synthesis and may attenuate negative energy balance. Circulating glucocorticoids (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) are increased during parturition in dairy cows and mediate differentiating and anti\u2010inflammatory effects via glucocorticoid (<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>) and mineralocorticoid receptors (<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content>) in the presence of the enzyme 11<jats:italic>\u03b2<\/jats:italic>\u2010hydroxysteroid dehydrogenase type 1 (11<jats:italic>\u03b2<\/jats:italic><jats:styled-content style=\"fixed-case\">HSD<\/jats:styled-content>1). Activated <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> are the main ligands for both receptors in <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>; therefore, we hypothesized that tissue\u2010specific <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> metabolism is effected by varying amounts of <jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MR<\/jats:styled-content> and 11<jats:italic>\u03b2<\/jats:italic><jats:styled-content style=\"fixed-case\">HSD<\/jats:styled-content>1 and\/or their localization within <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> depots. Furthermore, the lipolytic and antilipogenic effects of <jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content> might influence the <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> system in <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>. Therefore, we aimed to localize <jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> as well as the expression pattern and activity of 11<jats:italic>\u03b2<\/jats:italic><jats:styled-content style=\"fixed-case\">HSD<\/jats:styled-content>1 in different <jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> depots during early lactation in dairy cows and to identify potential effects of <jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content>. Primiparous German Holstein cows were divided into a control (<jats:styled-content style=\"fixed-case\">CON<\/jats:styled-content>) and a <jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content> group. From day 1 post\u2010partum (p.p.) until sample collection, the <jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content> group was fed with 100\u00a0g\/d <jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content> (contains 10\u00a0g each of the <jats:italic>cis<\/jats:italic>\u20109, <jats:italic>trans<\/jats:italic>\u201011 and the <jats:italic>trans\u2010<\/jats:italic>10, <jats:italic>cis\u2010<\/jats:italic>12\u2010<jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content> isomers). <jats:styled-content style=\"fixed-case\">CON<\/jats:styled-content> cows (<jats:italic>n<\/jats:italic>\u00a0=\u00a05 each) were slaughtered on day 1, 42 and 105 p.p., while <jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content> cows (<jats:italic>n<\/jats:italic>\u00a0=\u00a05 each) were slaughtered on day 42 and 105 p.p. Subcutaneous fat from tailhead, withers and sternum, and visceral fat from omental, mesenteric and retroperitoneal depots were sampled. The localization of <jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content> and 11<jats:italic>\u03b2<\/jats:italic><jats:styled-content style=\"fixed-case\">HSD<\/jats:styled-content>1 in mature adipocytes \u2013 being already differentiated \u2013 indicates that <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> promote other effects via <jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content> than differentiation. Moreover, <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> were observed in the stromal vascular cell fraction and positively related to the pre\u2010adipocyte marker Pref\u20101. However, only marginal <jats:styled-content style=\"fixed-case\">CLA<\/jats:styled-content> effects were observed in this study.<\/jats:p>","DOI":"10.1111\/jpn.12268","type":"journal-article","created":{"date-parts":[[2014,10,29]],"date-time":"2014-10-29T12:27:01Z","timestamp":1414585621000},"page":"950-961","source":"Crossref","is-referenced-by-count":3,"title":["Detection of 11 beta\u2010hydroxysteroid dehydrogenase type 1, the glucocorticoid and mineralocorticoid receptor in various adipose tissue depots of dairy cows supplemented with conjugated linoleic acids"],"prefix":"10.1111","volume":"99","author":[{"given":"K.","family":"Friedauer","sequence":"first","affiliation":[{"name":"Physiology and Hygiene Group Institute of Animal Science University of Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"D\u00e4nicke","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Friedrich\u2010Loeffler\u2010Institute (FLI) Federal Research Institute for Animal Health Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Schulz","sequence":"additional","affiliation":[{"name":"Physiology and Hygiene Group Institute of Animal Science University of Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Sauerwein","sequence":"additional","affiliation":[{"name":"Physiology and Hygiene Group Institute of Animal Science University of Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"H\u00e4ussler","sequence":"additional","affiliation":[{"name":"Physiology and Hygiene Group Institute of Animal Science University of Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7827(94)00006-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1807-59322011000500023"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2010-3868"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1992.tb16607.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1550191"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3037703"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2527\/2002.8051285x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.1092"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/reprod\/120.2.351"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)11222-8"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.7.6868"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-06-0042"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(89)79169-4"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-7970com"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-006-0107-9"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0250583"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.04.005"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(89)79081-0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2012.08.004"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.675355"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-125-4-2224"},{"volume-title":"Ausschuss f\u00fcr Bedarfsnormen der Gesellschaft f\u00fcr Ern\u00e4hrungsphysiologie. 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