{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:37:38Z","timestamp":1761709058205},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,1,10]],"date-time":"2015-01-10T00:00:00Z","timestamp":1420848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Higher Education Commission of Pakistan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Oxidative stress has detrimental effects on semen quality during spermatogenesis and semen processing for artificial insemination. This work was conducted to study the effect of different levels of vitamin E on the semen traits, oxidative status and trace minerals in Beetal bucks. Thirty\u2010six bucks of similar body weight and age (1\u00a0year) were randomly divided into four groups. One group was kept as control with no supplementation (group 1), and the others were supplemented with 200 (group 2), 400 (group 3) and 800\u00a0IU (group 4) vitamin E\/animal\/day for 2\u00a0months. At the end of the experiment, semen samples were collected and evaluated. Seminal plasma was separated to study the concentration of superoxide dismutase (<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>), glutathione peroxidase (<jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x), aspartate aminotransferase (<jats:styled-content style=\"fixed-case\">AST<\/jats:styled-content>), alanine aminotransferase (<jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>) and trace minerals (Zn, Cu, Mn and Fe). Group 3 showed significantly higher (p\u00a0&lt;\u00a00.05) semen volume and per cent motility and lower dead sperm percentage compared to control group. Superoxide dismutase, <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x, Zn, Cu and Mn were higher in the same group. The level of <jats:styled-content style=\"fixed-case\">AST<\/jats:styled-content> decreased in group 3 without any change on the concentration of <jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>. It is suggested that vitamin E at the rate of 400 <jats:styled-content style=\"fixed-case\">IU<\/jats:styled-content>\/buck\/day supported higher semen volume, per cent motility, per cent live spermatozoa, antioxidants (<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> GP<\/jats:styled-content>x) and trace mineral levels (Zn, Cu, Mn) in the seminal plasma. The increased supplementation from 0 to 400 showed a general increasing trend in improving semen quality. However, the dose of 800\u00a0IU\/kg had no useful effect in further improving the semen quality.<\/jats:p>","DOI":"10.1111\/jpn.12284","type":"journal-article","created":{"date-parts":[[2015,1,10]],"date-time":"2015-01-10T07:32:28Z","timestamp":1420875148000},"page":"841-846","source":"Crossref","is-referenced-by-count":16,"title":["<i>In vivo<\/i> adverse effects of alpha\u2010tocopherol on the semen quality of male bucks"],"prefix":"10.1111","volume":"99","author":[{"given":"A.","family":"Majid","sequence":"first","affiliation":[{"name":"Faculty of Animal Husbandry and Veterinary Sciences The University of Agriculture Peshawar Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. S.","family":"Qureshi","sequence":"additional","affiliation":[{"name":"Faculty of Animal Husbandry and Veterinary Sciences The University of Agriculture Peshawar Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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