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As there is limited literature on <jats:italic>in\u00a0vivo<\/jats:italic> biomarkers to assess increased <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> in chickens, the objective of this study was to identify a reliable biomarker of <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> using <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content> ingestion and fasting models. Male Ross chickens (<jats:italic>n<\/jats:italic>\u00a0=\u00a048) were reared until day 14 on the floor pen in an animal care facility, randomized into the following groups: control, <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content> and fasting (each with <jats:italic>n<\/jats:italic>\u00a0=\u00a016), and then placed in metabolism cages. <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content> was administered in drinking water at 0.75% from days 16 to 21, while controls and fasted groups received water. All birds had free access to feed and water except the birds in the fasting group that were denied feed for 19.5\u00a0h on day 20. On day 21, all chickens were given two separate oral gavages comprising fluorescein isothiocyanate dextran (<jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>\u2010d, 2.2\u00a0mg in 1\u00a0ml\/bird) at time zero and lactulose, mannitol and rhamnose (<jats:styled-content style=\"fixed-case\">LMR<\/jats:styled-content>) sugars (0.25\u00a0g L, 0.05\u00a0g M and 0.05\u00a0g R in 2\u00a0ml\/bird) at 60\u00a0min. Whole blood was collected from the brachial vein in a syringe 90\u00a0min post\u2010<jats:styled-content style=\"fixed-case\">LMR<\/jats:styled-content> sugar gavage. Serum <jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>\u2010d and plasma <jats:styled-content style=\"fixed-case\">LMR<\/jats:styled-content> sugar concentrations were measured by spectrophotometry and high\u2010performance ion chromatography respectively. Plasma concentrations of intestinal fatty acid binding protein, diamine oxidase, tight junction protein (<jats:styled-content style=\"fixed-case\">TJP<\/jats:styled-content>), <jats:sc>d<\/jats:sc>\u2010lactate and faecal <jats:italic>\u03b1<\/jats:italic>\u2010antitrypsin inhibitor concentration were also analysed by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>\u2010d increased significantly (p\u00a0&lt;\u00a00.05) after fasting compared with control. L\/M and L\/R ratios for fasting and L\/M ratio for <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content> increased compared with control chickens (p\u00a0&lt;\u00a00.05). <jats:styled-content style=\"fixed-case\">TJP<\/jats:styled-content> in plasma was significantly increased due to fasting but not <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content> treatment, compared with controls. Other tests did not indicate changes in <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> (p\u00a0&gt;\u00a00.05). We concluded that <jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>\u2010d and <jats:styled-content style=\"fixed-case\">LMR<\/jats:styled-content> sugar tests can be used in chickens to assess changes in <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jpn.12596","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T17:33:24Z","timestamp":1476380004000},"source":"Crossref","is-referenced-by-count":40,"title":["New biomarkers for increased intestinal permeability induced by dextran sodium sulphate and fasting in chickens"],"prefix":"10.1111","volume":"101","author":[{"given":"S.","family":"Gilani","sequence":"first","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide, Roseworthy Campus Adelaide SA Australia"},{"name":"Poultry CRC University of New England Armidale NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. S.","family":"Howarth","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide, Roseworthy Campus Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.","family":"Kitessa","sequence":"additional","affiliation":[{"name":"Commonwealth Scientific and Industrial Research Organisation, Health and Bio\u2010security Adelaide SA Australia"},{"name":"PPPI Nutrition Research Laboratory South Australian Research &amp; Development Institute Roseworthy SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. D.","family":"Tran","sequence":"additional","affiliation":[{"name":"Commonwealth Scientific and Industrial Research Organisation, Health and Bio\u2010security Adelaide SA Australia"},{"name":"School of Medicine Faculty of Health Sciences University of Adelaide Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. E. 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