{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:46:57Z","timestamp":1791974817131,"version":"4.4.0"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,10,16]],"date-time":"2016-10-16T00:00:00Z","timestamp":1476576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"DSM (China) Limited"},{"name":"China Pig Modern Industrial Technology System","award":["CARS-36"],"award-info":[{"award-number":["CARS-36"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This study was conducted to investigate the effects of benzoic acid (<jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>) on growth performance, intestinal development and intestinal barrier function in weaned pigs. Ninety weaned pigs were randomly assigned to one of three treatments: a basal diet (<jats:styled-content style=\"fixed-case\">CON<\/jats:styled-content>), the basal diet supplemented with 2000\u00a0mg\/kg benzoic acid (<jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>1) and 5000\u00a0mg\/kg benzoic acid (<jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>2). At the end of days 14 and 42, six pigs per treatment were randomly selected to collect plasma and intestinal samples. Results showed that <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content> supplementation not only improved final body weight, daily growth and feed conversion ratio from days 15 to 42 and days 1 to 42, but also decreased the activity of plasma diamine oxidase (day 42) and the <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> values of jejunal contents (day 14) (p\u00a0&lt;\u00a00.05). Ileal <jats:italic>Bacillus<\/jats:italic> populations (day 14) were increased by <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>, while <jats:italic>Escherichia coli<\/jats:italic> counts in the ileum and caecum (day 42) were decreased (p\u00a0&lt;\u00a00.05). Higher <jats:italic>Lactobacillus<\/jats:italic> counts occurred in the ileum (day 14, 42) of <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>1\u2010fed piglets as compared to <jats:styled-content style=\"fixed-case\">CON<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>2\u2010fed piglets (p\u00a0&lt;\u00a00.05). In addition, <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content> supplementation increased the ratio of villus height to crypt depth (day 14, 42) and decreased the crypt depth (day 14) (p\u00a0&lt;\u00a00.05). Growth\u2010stimulating factors (insulin\u2010like growth factor\u20101, day 42; insulin\u2010like growth factor\u20101 receptor, day 14, 42) and tight junction protein (occludin, day 14, 42; zonula occludens\u20101, day 42)\u2010related gene <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels were upregulated in the jejunum of piglets fed <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content> diets (p\u00a0&lt;\u00a00.05). In conclusion, this study provides the first evidence that <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content> has beneficial effects on intestinal development and intestinal barrier function of weaned pigs, which can partly explain why growth performance of pigs was improved by dietary <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content> supplementation.<\/jats:p>","DOI":"10.1111\/jpn.12627","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T03:27:33Z","timestamp":1476674853000},"page":"1137-1146","source":"Crossref","is-referenced-by-count":75,"title":["Benzoic acid beneficially affects growth performance of weaned pigs which was associated with changes in gut bacterial populations, morphology indices and growth factor gene expression"],"prefix":"10.1111","volume":"101","author":[{"given":"J. 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