{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T12:04:49Z","timestamp":1771589089127,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,1,8]],"date-time":"2017-01-08T00:00:00Z","timestamp":1483833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This study investigated the hypothesis that dietary concentrations of methionine (Met), as a precursor of cysteine which is a constituent of glutathione (<jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content>), affect tissue antioxidant concentrations and the antioxidant defence system in pigs. Forty\u2010five piglets (DanZucht\u00a0\u00d7\u00a0Pietrain) were allotted to three groups of similar mean body weight (11.0\u00a0\u00b1\u00a00.9\u00a0kg). The basal diet was composed of barley, wheat, corn starch, soybean oil, sucrose, cellulose and a mineral supplement with suboptimal concentrations of Met and was supplemented with <jats:sc>dl<\/jats:sc>\u2010Met to reach 0.16%, 0.20% and 0.24% of dietary Met and 0.40%, 0.44% and 0.48% of dietary Met and cysteine in groups 0.16, 0.20 and 0.24 respectively. After 3\u00a0weeks, at slaughter, samples of liver, jejunum mucosa and plasma were collected. Feed intake and weight gains increased and feed:gain ratio decreased when dietary Met concentrations increased. The Trolox equivalent antioxidant capacity (<jats:styled-content style=\"fixed-case\">TEAC<\/jats:styled-content>), concentrations of <jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content> and thiobarbituric acid reactive substances (<jats:styled-content style=\"fixed-case\">TBA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RS<\/jats:styled-content>) and the activity of the glutathione peroxidase (<jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x) in liver and jejunum mucosa were similar in all groups (p\u00a0&gt;\u00a00.05). Relative <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> concentrations of selected target genes of the nuclear factor (erythroid\u2010derived 2)\u2010like 2 (Nrf2), the master regulator of the antioxidant response, and of the nuclear factor \u2018kappa\u2010light\u2010chain\u2010enhancer\u2019 of activated B\u2010cells (<jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:italic>\u03ba<\/jats:italic>B), the master regulator of inflammation, were largely unaffected both in jejunum and liver. In conclusion, inflammation\u2010 and oxidative stress\u2010related pathways on the molecular level, and concentrations of lipid peroxidation products, of antioxidants and of enzymes involved in the antioxidant defence system were mostly unaffected by dietary Met concentration in gut and liver. These findings suggest that suboptimal dietary Met concentrations did not influence the antioxidant defence system of gut and liver in healthy piglets.<\/jats:p>","DOI":"10.1111\/jpn.12633","type":"journal-article","created":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T03:56:44Z","timestamp":1483934204000},"page":"1166-1174","source":"Crossref","is-referenced-by-count":14,"title":["Antioxidant status and expression of inflammatory genes in gut and liver of piglets fed different dietary methionine concentrations"],"prefix":"10.1111","volume":"101","author":[{"given":"J. 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