{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T21:14:25Z","timestamp":1770326065155,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,1,30]],"date-time":"2017-01-30T00:00:00Z","timestamp":1485734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education, Science and Technological Development, Republic of Serbia","award":["III 46002"],"award-info":[{"award-number":["III 46002"]}]},{"name":"Ministry of Education, Science and Technological Development, Republic of Serbia","award":["III41009"],"award-info":[{"award-number":["III41009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Thirty days before expected time of parturition, 20 Holstein cows were divided into \u2212Cr and +Cr groups. From day 25 before parturition (<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>) up to day 30 after parturition (<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>), +Cr cows received 10\u00a0mg of Cr (chromium\u2010enriched yeast) daily. Muscle and adipose tissue samples were taken at days \u221230, \u221210, +7 and +10 related to parturition, when body condition score (<jats:styled-content style=\"fixed-case\">BCS<\/jats:styled-content>) was also determined. Hepatic tissue samples were taken at days \u221210 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (<jats:styled-content style=\"fixed-case\">IVGTT<\/jats:styled-content>) was performed at days \u221228, \u22127, +10 and +30. Milk yield was recorded during first 14\u00a0weeks <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>. Milk composition was obtained at days 7 and 28 <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>. At day 10 <jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>, protein content of <jats:italic>\u03b2<\/jats:italic>\u2010subunit of insulin receptor (<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content><jats:italic>\u03b2<\/jats:italic>) was significantly higher (p\u00a0\u02c2\u00a00.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (<jats:styled-content style=\"fixed-case\">pIRS<\/jats:styled-content>\u20101 Ser<jats:sup>307<\/jats:sup>) was significantly lower (p\u00a0\u02c2\u00a00.05) in hepatic tissue of +Cr group. After parturition, <jats:styled-content style=\"fixed-case\">pIRS<\/jats:styled-content>\u20101 Ser<jats:sup>307<\/jats:sup> was significantly lower in muscle tissue at days 7 and 28 (p\u00a0\u02c2\u00a00.05 and p\u00a0\u02c2\u00a00.001, respectively), while phosphorylation of Akt at serine 473 (<jats:styled-content style=\"fixed-case\">pA<\/jats:styled-content>kt Ser<jats:sup>473<\/jats:sup>) was significantly higher (p\u00a0\u02c2\u00a00.01) in hepatic tissue at day 7 <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content> in +Cr group. Chromium had opposite effect on insulin kinetics during <jats:styled-content style=\"fixed-case\">IVGTT<\/jats:styled-content>s obtained <jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>. Insulin secretion was significantly reduced at day 7 <jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content> and significantly enhanced at day 10 <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>, when <jats:styled-content style=\"fixed-case\">NEFA<\/jats:styled-content> concentration was also significantly increased. Milk yield and <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> value were depressed in +Cr group. <jats:styled-content style=\"fixed-case\">DMI<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">BCS<\/jats:styled-content> were significantly enhanced in +Cr group at day 7 <jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post\u2010partal period, probably due to duration of exposure to chromium and different energy status between those periods.<\/jats:p>","DOI":"10.1111\/jpn.12655","type":"journal-article","created":{"date-parts":[[2017,1,30]],"date-time":"2017-01-30T08:49:23Z","timestamp":1485766163000},"page":"41-55","source":"Crossref","is-referenced-by-count":17,"title":["The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows"],"prefix":"10.1111","volume":"102","author":[{"given":"M.","family":"Panteli\u0107","sequence":"first","affiliation":[{"name":"Laboratory for Molecular Biology and Endocrinology Vin\u010da Institute of Nuclear Sciences University of Belgrade Belgrade Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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