{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:41:39Z","timestamp":1790530899109,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2018,2]]},"abstract":"Summary<\/jats:title>The present study was conducted to investigate the effects of different levels of dietary arginine (Arg) and methionine (Met) on performance, immune responses, and carcass characteristics of broiler chickens. A total of 540\u00a0day\u2010old Ross 308 broiler chicks were randomly assigned into the nine experimental diets, consisting five replicates of 12 birds each. Dietary treatments included three different levels (90%, 100%, and 110% of National Research Council [NRC<\/jats:styled-content>] specifications) of either dietary Arg or Met, which were fed to the birds according to a 3\u00a0\u00d7\u00a03 factorial arrangement of treatments during a 42\u00a0days feeding trial. Results showed that supplementation of Arg and Met into the deficient\u2010diets increased (p<\/jats:italic>\u00a0<\u00a0.01) weight gains during all trial periods. Although average daily feed intake (ADFI<\/jats:styled-content>) was not influenced by dietary treatments, increasing Arg up to 100% of NRC<\/jats:styled-content> recommendations improved (p<\/jats:italic>\u00a0<\u00a0.05) feed conversion ratio (FCR<\/jats:styled-content>) throughout the trial period. Similarly, supplementation of deficient\u2010diets with Met improved FCR<\/jats:styled-content> values. There was a significant (p<\/jats:italic>\u00a0<\u00a0.01) Arg\u00a0\u00d7\u00a0Met interaction for ADFI<\/jats:styled-content> during the starter period; increasing the dietary Arg level increased ADFI<\/jats:styled-content> when the diets were deficient in Met, while had an opposite effect in diets containing higher dietary Met levels. On the other hand, dietary Met fortification improved (p<\/jats:italic>\u00a0=\u00a0.067) FCR<\/jats:styled-content> values to a greater extent in 110% Arg\u2010diets during the entire trial period. Although different levels of Arg and Met had no marked effects on carcass yield and abdominal fat percentage, supplemental Arg up to 100% of NRC<\/jats:styled-content> values increased (p<\/jats:italic>\u00a0<\u00a0.01) the relative weights of spleen and bursa of Fabricius. Furthermore, bursa weight was affected by Arg\u00a0\u00d7\u00a0Met interaction (p<\/jats:italic>\u00a0<\u00a0.01), so that supplemental Arg level of 100% of NRC<\/jats:styled-content> increased the relative bursa weight in birds that were fed diets containing 90% and 110% of Met. Serum uric acid level was decreased (p<\/jats:italic>\u00a0<\u00a0.05) as a result of dietary Arg fortification up to 110% of NRC<\/jats:styled-content> recommended values. Supplementation of deficient\u2010diets with Met decreased (p<\/jats:italic>\u00a0<\u00a0.05) serum cholesterol level. Although Newcastle antibody titer was not affected by dietary Arg or Met levels, Arg fortification of deficient\u2010diets increased (p<\/jats:italic>\u00a0<\u00a0.001) antibody responses against infectious bronchitis (IBV<\/jats:styled-content>) and bursal (IBD<\/jats:styled-content>) disease viruses. Similarly, Met supplementation of deficient\u2010diets increased IBD<\/jats:styled-content> antibody titer. There were significant (p<\/jats:italic>\u00a0<\u00a0.05) Arg\u00a0\u00d7\u00a0Met interactions for IBV<\/jats:styled-content> and IBD<\/jats:styled-content> titers; Met fortification of 110% Arg\u2010diets was more effective in increasing antibody titers. An increase in dietary Met level up to 100% of NRC<\/jats:styled-content> values increased (p<\/jats:italic>\u00a0<\u00a0.001) serum concentration of \u03b3<\/jats:italic>\u2010globulins. The present findings imply that supplemental Arg could affect feed efficiency and antibody responses when the diets were already fortified with a sufficient Met level.<\/jats:p>","DOI":"10.1111\/jpn.12695","type":"journal-article","created":{"date-parts":[[2017,4,27]],"date-time":"2017-04-27T04:16:52Z","timestamp":1493266612000},"source":"Crossref","is-referenced-by-count":21,"title":["Marginal deficiencies of dietary arginine and methionine could suppress growth performance and immunological responses in broiler chickens"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9353-1120","authenticated-orcid":false,"given":"R.","family":"Jahanian","sequence":"first","affiliation":[{"name":"Poultry Nutrition Research Center Bioscitech Research Institute Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Khalifeh\u2010Gholi","sequence":"additional","affiliation":[{"name":"Poultry Nutrition Research Center Bioscitech Research Institute Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2006.121.127"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S0021-9258(18)72782-8","article-title":"Creatine formation in the chick","volume":"141","author":"Almquist H. J.","year":"1941","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_5_4_1","volume-title":"Official methods of analysis","author":"Association of Official Analytical Chemists","year":"2002"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199504000-00003"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1079\/9780851995830.0000"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/82.12.1932"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1079\/BJN2002732","article-title":"Dietary interrelationships among arginine, methionine, and lysine in young broiler chicks","volume":"88","author":"Chamruspollert M.","year":"2002","journal-title":"British Journal of Nutrition"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2015.09.019"},{"issue":"12","key":"e_1_2_5_10_1","first-page":"20","article-title":"Role of arginine in immune nutrition","volume":"35","author":"Efron D. T.","year":"2000","journal-title":"Journal of Gastroenterology"},{"key":"e_1_2_5_11_1","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1093\/jn\/58.4.459","article-title":"Growth and creatine biosynthesis in the chick as affected by the amino acid deficiencies of casein","volume":"58","author":"Fisher H.","year":"1956","journal-title":"The Journal of Nutrition"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4832-3210-2.50009-1"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2008-00386"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2008.70699"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2013.12.002"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1093\/jn\/101.2.217","article-title":"Relationship of arginine and methionine in the nutrition of the chicks and the significance of creatine biosynthesis in their interaction","volume":"10","author":"Keshavarz K.","year":"1971","journal-title":"The Journal of Nutrition"},{"issue":"1","key":"e_1_2_5_17_1","first-page":"1","article-title":"Immune functions as affected by dietary arginine by methionine interaction in broiler chicks","volume":"68","author":"Khalifeh\u2010Gholi M.","year":"2012","journal-title":"World's Poultry Science Journal"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1096\/fasebj.8.6.7909525","article-title":"Effect of glutathione depletion and oral N\u2010acetyl\u2010cysteine treatment on CD4+ and CD8+ cells","volume":"8","author":"Kinscherf R.","year":"1994","journal-title":"The FASEB Journal"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/78.11.1536"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2016.11.002"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/81.6.793"},{"key":"e_1_2_5_23_1","volume-title":"Scott's nutrition of the chickens","author":"Leeson S.","year":"2001"},{"key":"e_1_2_5_24_1","volume-title":"Commercial poultry nutrition","author":"Leeson S.","year":"2005"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2009.80473"},{"key":"e_1_2_5_26_1","volume-title":"Nutrient requirements of poultry","author":"National Research Council","year":"1994"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.24.8.735"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.243.5.C212"},{"key":"e_1_2_5_29_1","first-page":"142","article-title":"Performance of broiler chicken in early life on methionine deficient feed with added choline and betaine","volume":"2","author":"Rafeeq M.","year":"2011","journal-title":"International Journal of Livestock Production"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/0007166031000085283"},{"key":"e_1_2_5_31_1","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1017\/S1751731115000853","article-title":"Improved performance and immunological responses as the result of dietary genistein supplementation of broiler chicks","volume":"9","author":"Rasouli E.","year":"2015","journal-title":"Animal"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(05)80017-0"},{"key":"e_1_2_5_33_1","volume-title":"SAS statistics user's guide. Statistical analytical system","author":"SAS Institute","year":"1999"},{"key":"e_1_2_5_34_1","first-page":"746","article-title":"Methionine requirement and cell\u2010mediated immunity in chicks","volume":"94","author":"Shini S.","year":"2005","journal-title":"British Journal of Nutrition"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1983.572402x"},{"key":"e_1_2_5_36_1","volume-title":"Principles and procedures of statistics: A biometrical approach","author":"Steel R. G. D.","year":"1997"},{"key":"e_1_2_5_37_1","first-page":"52","article-title":"l\u2010Arginine stimulates immune response in chickens immunized with intermediate plus strain of infectious bursal disease vaccine","volume":"24","author":"Tayade C.","year":"2006","journal-title":"Vaccine"},{"key":"e_1_2_5_38_1","volume-title":"Veterinary immunology","author":"Tizard I. R.","year":"2012"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3360001"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.12695","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.12695","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T19:45:42Z","timestamp":1694979942000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.12695"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,26]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/jpn.12695"],"URL":"https:\/\/doi.org\/10.1111\/jpn.12695","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,26]]}}}