{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:23:56Z","timestamp":1785774236637,"version":"3.56.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T00:00:00Z","timestamp":1494806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"crossref","award":["2016YFD0500507"],"award-info":[{"award-number":["2016YFD0500507"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005064","name":"Science and Technology Support Program of Hubei Province","doi-asserted-by":"crossref","award":["2014BBA205"],"award-info":[{"award-number":["2014BBA205"]}],"id":[{"id":"10.13039\/501100005064","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Independent Scientific and Technological Innovation of Huazhong Agricultural University","award":["2662016PY010"],"award-info":[{"award-number":["2662016PY010"]}]},{"name":"State Key Laboratory of Animal Nutrition Open Project","award":["2004DA125184F1417"],"award-info":[{"award-number":["2004DA125184F1417"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:label\/><jats:p>The aim of this study was to examine the effects of different sources and levels of methionine (Met) on Heat shock proteins <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 expression and protein carbonylation in liver, <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 expression and malondialdehyde (<jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>) concentration in intestine under heat stress conditions during summer. A total of 720 (4\u00a0days old) Peking ducks were placed 20 per pen into six replicates for each of the six treatments with a 2\u00a0\u00d7\u00a03 factorial arrangement, such that two sources of Met (<jats:styled-content style=\"fixed-case\">DL<\/jats:styled-content>\u2010methionine [<jats:styled-content style=\"fixed-case\">DLM<\/jats:styled-content>] and <jats:styled-content style=\"fixed-case\">DL<\/jats:styled-content>\u20102\u2010hydroxy\u20104\u2010methylthiobutyrate [<jats:styled-content style=\"fixed-case\">HMTBA<\/jats:styled-content>] were supplemented at three different levels (0.05%, 0.20%, or 0.35% on as\u2010fed basis respectively). The experiment was divided into a starter (day 4\u201316) and a grower (day 17\u201335) period. Diet supplemented with 0.35% Met significantly up\u2010regulated the <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in duodenum, jejunum and ileum on day 16 and 35 as well as in liver on day 35 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) of ducks. <jats:styled-content style=\"fixed-case\">HMTBA<\/jats:styled-content>\u2010supplemented diets increased the <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in duodenum, jejunum, ileum and liver on day 35 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01). An increased <jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content> concentration was detected in jejunum of birds in 0.35% <jats:styled-content style=\"fixed-case\">DLM<\/jats:styled-content>\u2010supplemented treatment on day 16 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). And decreased protein carbonylation concentration was found in <jats:styled-content style=\"fixed-case\">DLM<\/jats:styled-content>\u2010supplemented treatment on day 16 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01). The birds fed with 0.35% Met supplemental diet displayed lower hepatic protein carbonylation on day 16 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In conclusion, supplementation of 0.35% Met in the duck diet showed up\u2010regulated <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 expression in small intestine and liver, which may provide new perspective to the mechanism of Met function. At the same time, <jats:styled-content style=\"fixed-case\">DLM<\/jats:styled-content> supplemented in diet may ameliorate oxidative status of liver, while <jats:styled-content style=\"fixed-case\">HMTBA<\/jats:styled-content> supplementation may partially improve the intestinal oxidative status of Peking ducks.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jpn.12722","type":"journal-article","created":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T05:23:32Z","timestamp":1494825812000},"source":"Crossref","is-referenced-by-count":6,"title":["A comparison of two sources of methionine supplemented at different levels on heat shock protein 70 expression and oxidative stress product of Peking ducks subjected to heat stress"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7540-2341","authenticated-orcid":false,"given":"L.","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition and Feed Science College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Feed Science College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. F.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Feed Science College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Y.","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Feed Science College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q. S.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Feed Science College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. X.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Feed Science College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. 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