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Eighteen lactating dairy cows were randomly assigned to one of three groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a06) and fed identical concentrate and corn silage with different forages on dry matter basis: (i) 23% alfalfa hay and 7% Chinese wild rye hay (<jats:styled-content style=\"fixed-case\">AH<\/jats:styled-content>); (ii) 30% CS; and (iii) 30%<jats:styled-content style=\"fixed-case\">RS<\/jats:styled-content>. After the 14\u2010week feeding, a total of 18 cows were slaughtered to collect digesta from four representative organs, including rumen, omasum, duodenum and jejunum. The<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>profiles of ruminal microbial fraction were similar among the treatments, except for greater Arg in cows fed<jats:styled-content style=\"fixed-case\">RS<\/jats:styled-content>than in cows fed<jats:styled-content style=\"fixed-case\">AH<\/jats:styled-content>or<jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>. Most of the analysed<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>changed under different diets. Significant differences were found among the microbial fraction, rumen fluid and rumen digesta, with greater essential<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>in digesta than in microbial fraction or rumen fluid and greater essential<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>in microbial fraction than rumen fluid. Significant differences in individual<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>profiles of digesta and relevant fluid were found across the four representative digestive tract parts, including rumen, omasum, duodenum and jejunum, showing much lower Leu proportion in<jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">RS<\/jats:styled-content>than in<jats:styled-content style=\"fixed-case\">AH<\/jats:styled-content>in duodenal fluid. In summary, ruminal microbes may prefer using essential<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>, rather than non\u2010essential<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>. The<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>profile of ruminal microbes was constant except for Arg. The<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>composition of digesta across the four digestive tracts changed dramatically, which indicated differences in the ability and efficiency of<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>absorption. The lower duodenum absorbable Leu proportion in cows fed CS or RS indicated the shortage of Leu in CS or RS diets, which might also restrict the balanced<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>absorption.<\/jats:p>","DOI":"10.1111\/jpn.12749","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T09:39:01Z","timestamp":1497346741000},"page":"421-428","source":"Crossref","is-referenced-by-count":5,"title":["Effect of cereal straw and alfalfa hay diet on amino acid profile of gastrointestinal digesta in lactating dairy cows"],"prefix":"10.1111","volume":"102","author":[{"given":"B.","family":"Wang","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory for Dairy Cow Nutrition College of Animal Science and Technology Beijing University of Agriculture Beijing China"},{"name":"Feed Research Institute Chinese Academy of Agricultural Sciences Beijing China"},{"name":"MoE Key Laboratory of Molecular Animal Nutrition Institute of Dairy Science College of Animal Sciences Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Tu","sequence":"additional","affiliation":[{"name":"Feed Research Institute Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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