{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T22:46:51Z","timestamp":1784674011302,"version":"3.55.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T00:00:00Z","timestamp":1508112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Short\u2010term fasting for 4.5 and 9\u00a0hr has been demonstrated to increase intestinal permeability (<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>) in chickens. This study aimed to investigate the effects of 0, 4.5, 9 and 19.5\u00a0hr fasting on intestinal gene expression and villus\u2010crypt architecture of enterocytes in jejunal and ileal samples. On day 38, Ross\u2010308 male birds were fasted according to their group and then euthanised. Two separate intestinal sections (each 2\u00a0cm long, jejunum and ileum) were collected. One section was utilised for villus height and crypt depth measurements. The second section was snap\u2010frozen in liquid nitrogen for quantitative polymerase chain reaction (<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>) analysis of tight junction proteins (<jats:styled-content style=\"fixed-case\">TJP<\/jats:styled-content>) including claudin\u20101, claudin\u20103, occludin, zonula occludens (<jats:styled-content style=\"fixed-case\">ZO<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">ZO<\/jats:styled-content>\u20102), junctional adhesion molecules (<jats:styled-content style=\"fixed-case\">JAM<\/jats:styled-content>) and E\u2010cadherin. Additionally genes involved in enterocyte protection including glucagon\u2010like peptide (<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102), heat\u2010shock protein (<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>\u201070), intestinal alkaline phosphatase (<jats:styled-content style=\"fixed-case\">IAP<\/jats:styled-content>), mammalian target of rapamycin (<jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content>), toll\u2010like receptors (<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>\u20104), mucin (<jats:styled-content style=\"fixed-case\">MUC<\/jats:styled-content>\u20102), cluster differentiation (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\u201036) and fatty acid\u2010binding protein (<jats:styled-content style=\"fixed-case\">FABP<\/jats:styled-content>\u20106) were also analysed. Normally distributed data were analysed using one\u2010way analysis of variance <jats:styled-content style=\"fixed-case\">ANOVA<\/jats:styled-content>. Other data were analysed by non\u2010parametric one\u2010way <jats:styled-content style=\"fixed-case\">ANOVA<\/jats:styled-content>. Villus height and crypt depth were increased (<jats:italic>p\u00a0&lt;<\/jats:italic>\u00a0.05) only in the ileum after fasting for 4.5 and 9\u00a0hr compared with non\u2010fasting group. <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of claudin\u20103 was significantly reduced in the ileum of birds fasted for 9 and 19.5\u00a0hr, suggesting a role in <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> modulation. However, all other <jats:styled-content style=\"fixed-case\">TJP<\/jats:styled-content> genes examined were not statistically different from control. Nevertheless, ileal <jats:styled-content style=\"fixed-case\">FABP<\/jats:styled-content>\u20106 of all fasted groups was significantly reduced, which could possibly be due to reduced bile acid production during fasting.<\/jats:p>","DOI":"10.1111\/jpn.12808","type":"journal-article","created":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T08:51:20Z","timestamp":1508143880000},"source":"Crossref","is-referenced-by-count":45,"title":["Gene expression and morphological changes in the intestinal mucosa associated with increased permeability induced by short\u2010term fasting in chickens"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2785-6883","authenticated-orcid":false,"given":"S.","family":"Gilani","sequence":"first","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide  Adelaide SA Australia"},{"name":"Poultry CRC University of New England  Armidale NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. S.","family":"Howarth","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Nattrass","sequence":"additional","affiliation":[{"name":"South Australian Research and Development Institute University of Adelaide  Roseworthy SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.","family":"Kitessa","sequence":"additional","affiliation":[{"name":"South Australian Research and Development Institute University of Adelaide  Roseworthy SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Barekatain","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide  Adelaide SA Australia"},{"name":"South Australian Research and Development Institute University of Adelaide  Roseworthy SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. E. A.","family":"Forder","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. D.","family":"Tran","sequence":"additional","affiliation":[{"name":"Health and Biosecurity Commonwealth Scientific and Industrial Research Organisation  Adelaide SA Australia"},{"name":"Faculty of Health Sciences School of Medicine University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Hughes","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide  Adelaide SA Australia"},{"name":"South Australian Research and Development Institute University of Adelaide  Roseworthy SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.6588"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2016.03.015"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2015.01.012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nutrit\/nuv104"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0739-7240(02)00210-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.04.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2015.00014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.06029-11"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/86.7.1414"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2013-1871"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0b013e32833f1222"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2007-00222"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2011-02062"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1071\/AN15725"},{"key":"e_1_2_7_17_1","article-title":"New biomarkers for increased intestinal permeability induced by dextran sodium sulphate and fasting in chickens","author":"Gilani S.","year":"2016","journal-title":"Journal of Animal Physiology and Animal Nutrition"},{"key":"e_1_2_7_18_1","first-page":"1","article-title":"Reduced fasting periods increase intestinal permeability in chickens","author":"Gilani S.","year":"2017","journal-title":"Journal of Animal Physiology and Animal Nutrition"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000000916"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0883-9441(99)90017-5"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.08.030"},{"key":"e_1_2_7_22_1","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.2527\/jas.2014-7673","article-title":"Impact of acute water and feed deprivation events on growth performance, intestinal characteristics, and serum stress markers in weaned pigs","volume":"92","author":"Horn N.","year":"2014","journal-title":"Journal of Animal Science"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2015.10.007"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092230307"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520150218048"},{"key":"e_1_2_7_26_1","first-page":"1","article-title":"Protective Role of p70S6K in intestinal ischemia \/ reperfusion injury in mice","volume":"7","author":"Kechen B.","year":"2012","journal-title":"PLoS ONE"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1637\/10757-122313-Reg.1"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pev114"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2010.04.012"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/13632758408454839"},{"key":"e_1_2_7_31_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.5713\/ajas.15.0120","article-title":"Intestinal alkaline phosphatase: Potential roles in promoting gut health in weanling piglets and its modulation by feed additives \u2010 A review","volume":"29","author":"Melo A. D.","year":"2016","journal-title":"Asian\u2010Australasian Journal of Animal Sciences"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.597492"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-2045"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069014"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070215"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2014.12.004"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2008.09.010"},{"key":"e_1_2_7_38_1","unstructured":"Ross. (2014).308 Broiler nutrition specification. Retrieved fromhttp:\/\/en.aviagen.com\/assets\/Tech_Center\/Ross_Broiler\/Ross308BroilerNutritionSpecs2014-EN.pdf"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0000000000000211"},{"key":"e_1_2_7_40_1","first-page":"372","article-title":"Delayed post hatch feeding affects performance, intestinal morphology and expression pattern of nutrient transporter genes in egg type chickens","volume":"05","author":"Shinde A. S.","year":"2014","journal-title":"Journal of Nutrition & Food Sciences"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-7364-5-3-170"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.5.1520"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.9.1535"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003600050142"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pev111"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.27222"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccc.2010.04.006"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071669608417922"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071660050194902"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.2141\/jpsa.32.241"},{"key":"e_1_2_7_51_1","first-page":"40","article-title":"Formula diet alters small intestine morphology, microbial abundance and reduces VE\u2010cadherin and IL\u201010 expression in neonatal porcine model","volume":"16","author":"Yeruva L.","year":"2016","journal-title":"Gastroenterology"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-4226com"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.15.0415"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.12808","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.12808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T14:30:00Z","timestamp":1695047400000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.12808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,16]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/jpn.12808"],"URL":"https:\/\/doi.org\/10.1111\/jpn.12808","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,16]]}}}