{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T04:57:42Z","timestamp":1775019462066,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T00:00:00Z","timestamp":1521763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2017A030310096"],"award-info":[{"award-number":["2017A030310096"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004000","name":"Guangzhou Science and Technology Program key projects","doi-asserted-by":"publisher","award":["2014Y2\u201000121"],"award-info":[{"award-number":["2014Y2\u201000121"]}],"id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The objective of this trial was to test the effects of oxidative stress induced by a high dosage of dietary iron on intestinal lesion and the microbiological compositions in caecum in Chinese Yellow broilers. A total of 450 1\u2010day\u2010old male chicks were randomly allotted into three groups. Supplemental iron (0, 700 and 1,400\u00a0mg\/kg) was added to the basal diet resulting in three treatments containing 245, 908 and 1,651\u00a0mg\/kg Fe (measured value) in diet respectively. Each treatment consisted of six replicate pens with 25 birds per pen. Jejunal enterocyte ultrastructure was observed by transmission electron microscopy. The results showed that a high dosage of dietary iron induced oxidative stress in broilers. Dilated endoplasmic reticulum (<jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>), autophagosome formation of jejunal enterocytes and decreased villi were caused by this oxidative stress. Compared to the control, concentration of the malondialdehyde (<jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>) in jejunal mucosa in the 908 and 1,651\u00a0mg\/kg Fe groups increased by 180% (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) and 155% respectively (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01); activity of copper\u2010zinc superoxide dismutase (Cu\/Zn<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>) increased in jejunum (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01); and the concentration of plasma reduced glutathione (<jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content>) decreased by 34.9% (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) in birds fed 1,651\u00a0mg\/kg Fe. Gene expression of nuclear factor, erythroid\u2010derived 2\u2010like 2 (<jats:italic>Nrf2<\/jats:italic>) and zonula occludens\u20101 (<jats:italic><jats:styled-content style=\"fixed-case\">ZO<\/jats:styled-content>\u20101<\/jats:italic>), in the higher dietary Fe groups was enhanced (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Species of microbial flora in caecum increased caused by oxidative stress. The <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DGGE<\/jats:styled-content> (denaturing gradient gel electrophoresis) dendrograms revealed different microbiota (65% similarity coefficient) between the control and iron\u2010supplemented groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). These data suggest high dosage of iron supplement in feed diet can induce oxidative stress in Chinese Yellow broilers, and composition of microbiota in the caecum changed. It implied there should be no addition of excess iron when formulating diets in Chinese Yellow broilers.<\/jats:p>","DOI":"10.1111\/jpn.12885","type":"journal-article","created":{"date-parts":[[2018,3,24]],"date-time":"2018-03-24T06:20:23Z","timestamp":1521872423000},"page":"924-932","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Effects of oxidative stress induced by high dosage of dietary iron ingested on intestinal damage and caecal microbiota in Chinese Yellow broilers"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8258-6532","authenticated-orcid":false,"given":"Z. Y.","family":"Gou","sequence":"first","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal Science Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal Science Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q. L.","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal Science Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X. J.","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal Science Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z. Y.","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal Science Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. T.","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal Science Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. Y.","family":"Ding","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal Science Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9251-306X","authenticated-orcid":false,"given":"S. Q.","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture Guangdong Public Laboratory of Animal Breeding and Nutrition Guangdong Key Laboratory of Animal Breeding and Nutrition Institute of Animal Science Guangdong Academy of Agricultural Sciences  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071660310001618334"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-008-0311-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/114.7.1306"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.04.015"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-011-9163-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.111.148643"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520310000771"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0620085"},{"key":"e_1_2_6_10_1","volume-title":"Ultrastructural pathology of the cell and matrix: a text and atlas of physiological and pathological alterations in the fine structure of cellular and extracellular components","author":"Ghadially F. N.","year":"2013"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2002.tb11051.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033627"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2013.13744"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.100599"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2004.tb03620.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2006.01.032"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.11.2928"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.3.695-700.1993"},{"key":"e_1_2_6_19_1","volume-title":"Mineral tolerance of domestic animals","author":"National Research Council, NRC.","year":"1980"},{"key":"e_1_2_6_20_1","volume-title":"Nutrient requirements of poultry","author":"National Research Council, NRC.","year":"1994"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2001.00930.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2005.07.001"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.5525"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-012-4323-6"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.277.6.G1149"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0946-672X(97)80033-8"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/713654910"},{"key":"e_1_2_6_28_1","volume-title":"The effect of GLP\u20101 (Glucagon\u2010Like Peptide 1) on insulin sensitivity in diabetic dogs","author":"Sandhu H. S.","year":"1997"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1046"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3536-3542.2004"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2015.1004622"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-01-00190.1997"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00114.2005"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00422.2002"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(02)00361-2"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071668708416992"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1996.0089"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-012-9409-6"},{"key":"e_1_2_6_39_1","volume-title":"Feeding standard of chicken","author":"Wen J.","year":"2004"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.04.058"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.12885","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.12885","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T08:52:40Z","timestamp":1695027160000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.12885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,23]]},"references-count":39,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1111\/jpn.12885"],"URL":"https:\/\/doi.org\/10.1111\/jpn.12885","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,23]]},"assertion":[{"value":"2017-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}