{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T21:21:02Z","timestamp":1790544062063,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T00:00:00Z","timestamp":1538438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The influence of medium\u2010chain glycerides on performance and gastrointestinal well\u2010being in weaning piglets was assessed. First, caproic (C6), caprylic (C8) and capric (C10) acid activity against<jats:italic>Escherichia coli<\/jats:italic>was screened in vitro. Pig flora of the whole small intestine was used as inoculum. Seven in vitro incubations were done in duplicate at pH\u00a0=\u00a03 and 5: C10 (15\u00a0mM), C8 (12\u00a0mM), C6 (15, 12, 10\u00a0mM), a non\u2010incubated\u2010negative control and incubated negative control. Culture suspensions were plated on<jats:italic>E.\u00a0coli<\/jats:italic>\u2010selective agar. Controls showed bacterial growth. C6 and C8 showed no growth at both pH\u2010values, where C10 showed growth at pH\u00a0=\u00a05. Secondly, an in vivo study was done with 80 weaned piglets over 42\u00a0days, housed in pens of eight animals (five pens\/treatment), fed a basal diet containing broken rice\/soya bean meal\/fish meal and supplemented with C6 and C8 in medium\u2010chain glyceride form (MCT6\/8, 0.175%) or antibiotic growth promoter (AGP, 0.020%) (Kasetsart University, Thailand) serving as control. Feed intake, daily gain and feed\u2010to\u2010gain ratio did not differ between MCT6\/8 and AGP. Per replicate, two random selected piglets were challenged intravenously with<jats:italic>E.\u00a0coli<\/jats:italic>\u2010lipopolysaccharide (LPS) or saline solution (S) at Days 21 and 28. All challenged animals were sacrificed; blood and digestive tract samples (jejunum\/ileum) were collected at Day 35. LPS challenge consistently reduced villus height and crypt depth for MCT6\/8 and AGP. However, LPS\u2010challenged piglets supplemented with MCT6\/8 restored villus height, where AGP did not. MCT6\/8 piglets had higher serum IgA, more jejunal IgA\u2010positive plasma cells and goblet cells than AGP. At the ileal level, results were similar, though less pronounced. The present study offers new insight in the benefits of MCT6\/8 over AGP in the post\u2010weaning period. There is in vitro anti\u2010microbial action of C6 and C8 on<jats:italic>E.\u00a0coli<\/jats:italic>. In vivo, MCT6\/8 also has protective effects in the small intestine that may result in growth promotion.<\/jats:p>","DOI":"10.1111\/jpn.12998","type":"journal-article","created":{"date-parts":[[2018,10,3]],"date-time":"2018-10-03T03:58:17Z","timestamp":1538539097000},"page":"221-230","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Medium\u2010chain glycerides affect gut morphology, immune\u2010 and goblet cells in post\u2010weaning piglets: In vitro fatty acid screening with<i>Escherichia coli<\/i>and in vivo consolidation with LPS challenge"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9341-6739","authenticated-orcid":false,"given":"Kirsten","family":"De Keyser","sequence":"first","affiliation":[{"name":"AVEVE Biochem NV Merksem Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00ebl","family":"Dierick","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uthai","family":"Kanto","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture Kasetsart University Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tassanan","family":"Hongsapak","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture Kasetsart University Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goedele","family":"Buyens","sequence":"additional","affiliation":[{"name":"AVEVE Biochem NV Merksem Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leni","family":"Kuterna","sequence":"additional","affiliation":[{"name":"AVEVE Biochem NV Merksem Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Vanderbeke","sequence":"additional","affiliation":[{"name":"AVEVE Biochem NV Merksem Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,2]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Amcra Visie 2020","author":"AMCRA","year":"2014"},{"key":"e_1_2_6_3_1","volume-title":"AOAC","author":"AOAC International","year":"2005"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1005.030735"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-014-0396-x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2527\/2004.8261764x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.05.008"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2005.12.006"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2014.0037"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2012.03.001"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.05.001"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1079\/NRR200369"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0377-8401(02)00224-9"},{"key":"e_1_2_6_14_1","first-page":"49","article-title":"The combined use of whole Cuphea seeds containing medium chain fatty acids and an exogenous lipase in piglet nutrition","volume":"57","author":"Dierick N. A.","year":"2003","journal-title":"Archiv F\u00fcr Tierern\u00e4hrung"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-6226(01)00303-7"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-6226(01)00331-1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12225"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2016.08.002"},{"key":"e_1_2_6_19_1","unstructured":"Van DerHeyde H. &Henderickx H.(1963).Vereinfachung der quantitativen und qualitativen Bestimmung der Bakterien unter Verwendung von \u2018Ringplatten\u2019. Zentralblatt Bakteriol I Orig. 189 224\u2013228."},{"key":"e_1_2_6_20_1","volume-title":"Dietary manipulation of infectious bowel disease. Biology of Growing Animals","author":"Hopwood D. E.","year":"2006"},{"key":"e_1_2_6_21_1","first-page":"271","article-title":"The antimicrobial function of milk lipids","volume":"10","author":"Isaacs C. E.","year":"2001","journal-title":"Advances in Nutritional Research"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-008-2308-0"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","first-page":"471","DOI":"10.2460\/ajvr.1990.51.03.471","article-title":"Effect of orally administered epidermal growth factor on the jejunal mucosa of weaned pigs","volume":"51","author":"Jaeger L. A.","year":"1990","journal-title":"American Journal of Veterinary Research"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-011-0822-3"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00457.2003"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.12.012"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.08.002"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.618595"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2006-153"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/126.4.933"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12182"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-6226(97)00057-2"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2014.05.007"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00047.2013"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2015.1086057"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1993.1090"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02932141"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02931402"},{"key":"e_1_2_6_39_1","volume-title":"Guide for the care and use of agricultural animals in research and teaching","author":"The Federation of Animal Science Societies","year":"2010"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.6.3582-3587.2004"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/japr\/14.1.122"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1301975110"},{"key":"e_1_2_6_43_1","first-page":"A15","volume-title":"Improving food safety through a one health approach: Workshop Summary","author":"Wegener H. C.","year":"2012"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2004.01958.x"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252311000089"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.12998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.12998","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpn.12998","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.12998","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T23:47:40Z","timestamp":1720655260000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.12998"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,2]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/jpn.12998"],"URL":"https:\/\/doi.org\/10.1111\/jpn.12998","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,2]]},"assertion":[{"value":"2018-04-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}