{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:43:35Z","timestamp":1774439015775,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T00:00:00Z","timestamp":1539302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"CJ Europe, Schwalbach am Taunus, Germany"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study investigated the hypothesis that dietary supplementation of L\u2010methionine (L\u2010Met) in weaned piglets in relation to DL\u2010methionine (DL\u2010Met) results in a higher antioxidant status and lower need for antioxidant enzyme activation in intestinal epithelium and body tissues, and improves gut morphology and gut barrier function as well as performance. A total of 99 early\u2010weaned 21\u2010day old piglets were allotted to six groups and fed a semi\u2010synthetic wheat\u2013barley\u2010based basal diet supplemented with 0.067%, 0.107% and 0.147% of either DL\u2010Met (MetAmino; Evonik, Hanau, Germany) or L\u2010Met (L\u2010Met100; CJ Europe, Schwalbach am Taunus, Germany) to reach dietary Met concentrations of 0.16%, 0.20% and 0.24%, of which the latter met the requirements for maintenance and growth based on a pre\u2010experiment. Feed intake and body weights were recorded weekly, and samples of plasma, liver and duodenum and jejunum mucosa were collected after 3\u00a0weeks at slaughter. Plasma concentrations of L\u2010Met were similar, and those of D\u2010Met and total Met were higher in piglets fed DL\u2010Met in relation to those fed L\u2010Met. Feed intake, daily gains and feed:gain ratio, and the relative bio\u2010efficacy based on gains and feed:gain ratio were similar for both groups. Likewise, villi length, crypt depth, the villi length:crypt depth ratio in duodenum and jejunum and gene expression of tight junction proteins in the jejunum did not differ. Concentrations of antioxidants like glutathione and tocopherol, the total antioxidant capacity, the mRNA abundance or activity of antioxidant enzymes like superoxide dismutase and glutathione peroxidase, concentrations of thiobarbituric acid reactive substances, markers for oxidative damage of lipids and the expression of inflammatory genes were similar in liver and jejunum mucosa. These data indicate that the effects of L\u2010Met and DL\u2010Met supplementation are comparable considering both piglet performance and parameters of gut health and function like gut morphology and the intestinal antioxidant status.<\/jats:p>","DOI":"10.1111\/jpn.13000","type":"journal-article","created":{"date-parts":[[2018,10,13]],"date-time":"2018-10-13T04:45:05Z","timestamp":1539405905000},"page":"242-250","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Effects of L\u2010methionine on performance, gut morphology and antioxidant status in gut and liver of piglets in relation to DL\u2010methionine"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4559-4036","authenticated-orcid":false,"given":"Johanna O.","family":"Zeitz","sequence":"first","affiliation":[{"name":"Institute of Animal Nutrition and Nutritional Physiology University of Giessen Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Kaltenb\u00f6ck","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Nutritional Physiology University of Giessen Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Most","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Nutritional Physiology University of Giessen Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3246-4136","authenticated-orcid":false,"given":"Klaus","family":"Eder","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition and Nutritional Physiology University of Giessen Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.91021.2008"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9584932"},{"key":"e_1_2_8_4_1","first-page":"509","article-title":"Regional differences for the d\u2010amino acid oxidase\u2010catalysed oxidation of d\u2010methionine in chicken small intestine","volume":"101","author":"Brachet P.","year":"1992","journal-title":"Comparative Biochemistry and Physiology"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2013.08.007"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-014-1675-5"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4141\/cjas92-024"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(03)00003-2"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0710700"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-55-18"},{"key":"e_1_2_8_11_1","volume-title":"Empfehlungen zur Energie\u2010 und N\u00e4hrstoffversorgung von Schweinen","author":"GfE (Gesellschaft f\u00fcr En\u00e4hrungsphysiologie)","year":"2006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4002"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90139-6"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00407.2004"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.8.2001"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.02.068"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2015.08.001"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/nure.12050"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-011-0962-7"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02490453"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2368"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(76)90747-6"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2008.05.005"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2695"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1017\/S000711450720580X"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.178"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D032698"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.230"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607965104"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/0049825031000150444"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2015-8898"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-7830"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.7.1609"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/128.3.606"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.15.0730"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(69)90064-5"},{"issue":"7","key":"e_1_2_8_37_1","article-title":"Polyamines and gut mucosal homeostasis","volume":"2","author":"Timmons J.","year":"2014","journal-title":"Journal of Gastrointestinal & Digestive System"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.07.087"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-8230"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.08.008"},{"key":"e_1_2_8_41_1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/clinchem\/33.2.214","article-title":"Lipoperoxides in plasma as measured by liquid\u2010chromatographic separation of malondialdehyde\u2010thiobarbituric acid adduct","volume":"33","author":"Wong S. H.","year":"1987","journal-title":"Clinical Chemistry"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.12633"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.13000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpn.13000","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T18:00:41Z","timestamp":1694196041000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.13000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,12]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/jpn.13000"],"URL":"https:\/\/doi.org\/10.1111\/jpn.13000","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,12]]},"assertion":[{"value":"2018-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}