{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T20:27:14Z","timestamp":1784752034704,"version":"3.55.0"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this study was to verify the ability of a probiotic in the feed to maintain the stability of the gut microbiota in chickens after antibiotic therapy and its association with growth performance. One thousand six hundred twenty 1\u2010day\u2010old Cobb male were housed in floor pens (36 pens, 45 birds\/pen) and were fed corn\u2010\/soya bean meal\u2010based diets supplemented with or without probiotic (<jats:italic>Bacillus subtilis<\/jats:italic>) during the entire rearing phase. From 21 to 24\u00a0days of age (three consecutive days), the chickens were submitted to antibiotic therapy via drinking water (bacitracin and neomycin) in order to mimic a field treatment and induce dysbiosis. Growth performance was monitored until 42\u00a0days of age. At 2, 4 and 6\u00a0days after antibiotic therapy, three chickens from each pen were euthanized and the contents of the small intestine and caeca were collected and pooled. The trial was conducted with four treatments and nine replicates in a 2\u00a0\u00d7\u00a02 factorial arrangement for performance characteristics (with and without probiotic\u00a0\u00d7\u00a0with and without antibiotic therapy); for the intestinal microbiota, it was in a 2\u00a0\u00d7\u00a02\u00a0\u00d7\u00a03 factorial arrangement (with and without probiotic\u00a0\u00d7\u00a0with and without antibiotic therapy\u00a0\u00d7\u00a02, 4 and 6\u00a0days after the antibiotic therapy) with three replicates per treatment. Terminal restriction length polymorphism (T\u2010RFLP) analysis showed that the structure of gut bacterial community was shaped by the intestinal segment and by the time after the antibiotic therapy. The number of 16S rDNAs copies in caecum contents decreased with time after the therapeutic treatment. The antibiotic therapy and dietary probiotic supplementation decreased richness and diversity indexes in the caecal contents. The improved performance observed in birds supplemented with probiotic may be related to changes promoted by the feed additive in the structure of the intestinal bacterial communities and phylogenetic groups. Antibiotic therapy modified the bacterial structure, but did not cause loss of broiler performance.<\/jats:p>","DOI":"10.1111\/jpn.13004","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T17:52:08Z","timestamp":1543427528000},"page":"72-86","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Performance and intestinal microbiota of chickens receiving probiotic in the feed and submitted to antibiotic therapy"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2365-845X","authenticated-orcid":false,"given":"Rafaela","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Animal Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiano","family":"Bortoluzzi","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ademir","family":"Durrer","sequence":"additional","affiliation":[{"name":"Department of Soil Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naiara Simarro","family":"Fagundes","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Ayres","family":"Pedroso","sequence":"additional","affiliation":[{"name":"Lina Bioscience Visalia California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaqueline Moreira","family":"Rafael","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falia Elidia de Lima","family":"Perim","sequence":"additional","affiliation":[{"name":"Department of Soil Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelen Cristiane","family":"Zavarize","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glaucia Samira","family":"Napty","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Dini","family":"Andreote","sequence":"additional","affiliation":[{"name":"Department of Soil Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diogo Paes","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Soil Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Fernando Machado","family":"Menten","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Luiz de Queiroz College of Agriculture University of Sao Paulo Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091213-113052"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010463"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"4084","DOI":"10.1128\/AEM.64.10.4084-4088.1998","article-title":"Effective recovery of bacterial DNA and percent\u2010guanosine\u2010plus\u2010cytosine based analysis of community structure in the gastrointestinal tract of broiler chickens","volume":"64","author":"Apajalahti J. H. A.","year":"1998","journal-title":"Applied and Environmental Microbiology"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166133"},{"key":"e_1_2_7_6_1","unstructured":"Bell J.(2011).Overview of tailed amplicon sequencing approach with MiSeq Illumina USA."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2015.06.006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05100-11"},{"key":"e_1_2_7_9_1","first-page":"335","article-title":"QIIME allows analysis of high\u2010throughput community sequencing data","volume":"7","author":"Caporaso J. G.","year":"2010","journal-title":"Nature"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4238\/2013.May.23.1"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731114002584"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pev329"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.27128"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1079\/PNS19840027"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2008.04116.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2014.899430"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/japr\/14.4.750"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2008.02408.x"},{"key":"e_1_2_7_19_1","first-page":"1","article-title":"PAST: Paleontological statistics software package for education and data analysis","volume":"4","author":"Hammer O.","year":"2001","journal-title":"Paleontol Electron Journal"},{"key":"e_1_2_7_20_1","first-page":"506","article-title":"The international Scientific Association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic","volume":"11","author":"Hill C.","year":"2014","journal-title":"Nature Reviews"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2003.tb01040.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/japr\/12.4.461"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.03.003"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1964.03070110019004"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2012-02528"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2009-00464"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/4441022a"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188634"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2004.09.015"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.11.6816-6824.2003"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12399"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071668.2017.1307322"},{"key":"e_1_2_7_33_1","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/aem.59.3.695-700.1993","article-title":"Profiling of complex microbial\u2010populations by denaturing gradient gel\u2010electrophoresis analysis of polymerase chain reaction\u2010 amplified genes\u2010coding for 16S ribosomal\u2010RNA","volume":"59","author":"Muyzer G.","year":"1993","journal-title":"Environmental Microbiology"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2015.00075"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.26945"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/82.4.627"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.04.004"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.4.658-672.2003"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.1.4.12614"},{"key":"e_1_2_7_40_1","volume-title":"Brazilian tables for poultry and swine \u2013 Composition of feedstuffs and nutritional requirements","author":"Rostagno H. S.","year":"2011"},{"key":"e_1_2_7_41_1","volume-title":"SAS User\u2019s Guide. Version 9.1 ed","author":"SAS","year":"2006"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.71"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2010-00745"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2012-02603"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1947-7"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13353-013-0179-4"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg346"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2010.543414"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1051\/animres:19980402"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(00)00227-2"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1081\/ABIO-120005774"},{"key":"e_1_2_7_52_1","first-page":"1","article-title":"Chicken faecal microbiota and disturbances induced by single or repeat therapy with tetracycline and streptomycin","volume":"9","author":"Videnska P.","year":"2013","journal-title":"Veterinary Research"},{"key":"e_1_2_7_53_1","first-page":"1","article-title":"Role of probiotics in immune function and their relationship to antibiotic growth promoters in poultry, a brief review","volume":"11","author":"Vuong C. N.","year":"2016","journal-title":"International Journal of Probiotics and Prebiotics"},{"key":"e_1_2_7_54_1","first-page":"5","article-title":"Characterising the avian gut microbiota: Membership, driving influences and potential function","volume":"16","author":"Waite D. W.","year":"2014","journal-title":"Frontiers in Microbiology"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/86.9.1856"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0043933909000087"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03412"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03314"},{"key":"e_1_2_7_59_1","first-page":"1","article-title":"Quantitative genetic background of the host influences gut microbiomes in chickens","volume":"3","author":"Zhao L.","year":"2013","journal-title":"Scientific Reports"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.1.124-137.2002"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.13004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpn.13004","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T18:00:50Z","timestamp":1694196050000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.13004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,28]]},"references-count":59,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/jpn.13004"],"URL":"https:\/\/doi.org\/10.1111\/jpn.13004","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,28]]},"assertion":[{"value":"2017-07-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}