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One\u2010day\u2010old Arbor Acre (<jats:italic>n<\/jats:italic>\u00a0=\u00a0480) were randomly assigned to four treatments with 10 cages of 12 birds: (a) basal diet negative control (NC), with no probiotics nor antibiotics formulated to contain 2,930 and 3,060\u00a0kcal\/kg with 24.07 and 15.98% CP, for starter and finisher diet, respectively, (b) basal diet\u00a0+\u00a0enramycin (5\u00a0mg\/kg), an antibiotic growth promoter (AGP); (c) basal diet\u00a0+\u00a0<jats:italic>Bacillus subtilis<\/jats:italic> B21 at 2 \u00d7 10<jats:sup>9 <\/jats:sup>CFU per g (BS); (d) basal diet\u00a0+\u00a0<jats:italic>Bacillus licheniformis<\/jats:italic> B26 at 2 \u00d7 10<jats:sup>9 <\/jats:sup>CFU per g (BL); growth performance, intestinal morphology, intestinal lesion scores, short\u2010chain fatty acids (SCFAs) and mucosal barrier tight junction's (TJ) mRNA expression were assessed. NC\u2010 and BL\u2010fed groups showed higher (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.005) average daily feed intake from d1 to d21 than AGP and BS, whereas BS\u2010 and AGP\u2010fed groups showed higher average daily weight gain from d22 to d42 and d1 to d42 of age. Higher mortality rate of (12.5%) and lower of (5.5%) were recorded in AGP and NC fed\u2010groups respectively, lesion score was higher in BS and BL than in AGP, while no lesion was observed in NC group, results revealed higher duodenum and jejunum villus height to crypt depth (VH:CD) compared with NC and BS. Probiotics\u2010fed groups showed higher total (SCFAs), acetic and butyric acid concentrations at d21 post\u2010challenge (PC) than other groups. The expression of claudin\u20101 was upregulated in duodenum (d7) PC and in jejunum (d7) and (d21) PC in BL group, while at d21 PC, the expression of occludens was higher in jejunum and ileum by AGP and BL. The present study indicated both BS and BL have some similarity with AGP in preventing or partially preventing NE effect in broilers.<\/jats:p>","DOI":"10.1111\/jpn.13082","type":"journal-article","created":{"date-parts":[[2019,4,24]],"date-time":"2019-04-24T06:10:33Z","timestamp":1556086233000},"page":"1039-1049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["<i>Bacillus subtilis<\/i> B21 and <i>Bacillus licheniformis<\/i> B26 improve intestinal health and performance of broiler chickens with <i>Clostridium perfringens<\/i>\u2010induced necrotic enteritis"],"prefix":"10.1111","volume":"103","author":[{"given":"Bello Bodinga","family":"Musa","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"},{"name":"Shehu Shagari College of Education Sokoto Sokoto State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongle","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayat","family":"Khawar","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingzhu","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouzheng","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Abdalla","family":"Elsiddig Mohamed","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imtiaz Hussain Raja","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition, Faculty of Animal Production and Technology Cholistan University of Veterinary and Animal Sciences Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9702-7039","authenticated-orcid":false,"given":"Xiaojun","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,23]]},"reference":[{"issue":"6","key":"e_1_2_8_2_1","first-page":"978","article-title":"Bacillus subtilis PB6 based\u2010probiotic (CloSTATTM) improves intestinal morphological and microbiological status of broiler chickens under Clostridium perfringens challenge","volume":"15","author":"Abudabos A. 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