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The aim of this study was to investigate the effects of EB on the barrier function in the intestinal porcine epithelial cells J2 (IPEC\u2010J2). The IPEC\u2010J2 cells were inoculated into 96\u2010well plates at a density of 5\u00a0\u00d7\u00a010<jats:sup>3<\/jats:sup> cells per well for 100% confluence. The cells were cultured in the presence of EB at concentrations of 0, 0.05, 0.10, and 0.20\u00a0mg\/ml for 48\u00a0hr. Then, 0.10\u00a0mg\/ml was selected as the suitable concentration for the estimation of transepithelial electric resistance (TEER) value, alkaline phosphatase activity, proinflammatory cytokines mRNA expression, tight junction mRNA and protein expression. The results of this study indicated that the supplementation of EB in IPEC\u2010J2 cells decreased cellular membrane permeability and mRNA expression of proinflammatory cytokines, including interleukin\u20106 (IL\u20106), interferon\u2010\u03b3 (INF\u2010\u03b3), and tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1). The supplementation of EB in IPEC\u2010J2 cells increased tight junction protein expression and anti\u2010inflammatory cytokines, interleukin 10 (IL\u201010) and transforming growth factor beta (TGF\u2010\u03b2). In addition, the western blotting and real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR) results indicated that EB significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) increased the mRNA and protein expression of intestinal tight junction proteins, Claudin\u20103, Occludin, and Zonula Occludins protein\u20101 (ZO\u20101). Therefore, dietary supplementation of EB may increase intestinal barrier function, tight junction protein expression, anti\u2010inflammatory cytokines, and decrease proinflammatory cytokines synthesis in IPEC\u2010J2 cells.<\/jats:p>","DOI":"10.1111\/jpn.13087","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T16:52:49Z","timestamp":1555433569000},"page":"1174-1184","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Eleutheroside B increase tight junction proteins and anti\u2010inflammatory cytokines expression in intestinal porcine jejunum epithelial cells (IPEC\u2010J2)"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3632-2469","authenticated-orcid":false,"given":"Dongsheng","family":"Che","sequence":"first","affiliation":[{"name":"Jilin Provincial Key Lab of Animal Nutrition and Feed Science Jilin Agricultural University Changchun 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