{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:02:52Z","timestamp":1769904172291,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["141453"],"award-info":[{"award-number":["141453"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["2012-5"],"award-info":[{"award-number":["2012-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study was conducted to verify how feed restriction affects gut microbiota and gene hepatic expression in broiler chickens and how these variables are related to body weight gain. For the experiment, 21\u2010d\u2010old Cobb500<jats:sup>TM<\/jats:sup> birds were distributed in a completely randomized experimental design with three treatments: T1. Control (ad libitum\u20143.176\u00a0Mcal\/kg ME\u2014metabolizable energy\u2014and 19% CP\u2014crude protein); T2. Energetic restriction (2.224\u00a0Mcal\/kg ME and 19% CP) from 22 to 42\u00a0days with consumption equivalent to control; T3. Quantitative restriction (70% restriction, i.e., restricted broilers ingested only 30% of the quantity consumed by the control group\u20143.176\u00a0Mcal\/kg ME and 19% CP) for 7\u00a0days, followed by refeeding ad libitum from 28 to 42\u00a0days. Ileum and caecum microbiota collections were made at 21, 28 and 42\u00a0days of age. Hepatic tissue was collected at 28 and 42\u00a0days old for relative gene expression analyses. At 43\u2010d\u2010old, body composition was quantified by DXA (Dual\u2010energy X\u2010ray Absorptiometry). Both feed restriction programmes decreased <jats:italic>Lactobacillus <\/jats:italic>and increased <jats:italic>Enterococcus<\/jats:italic> and <jats:italic>Enterobacteriaceae<\/jats:italic> counts. No differences were found in the refeeding period. Energetic restriction induced the expression of <jats:italic>CPT1\u2010A<\/jats:italic> (Carnitine palmitoyltransferase 1A) gene, and decreased body fat mass. Quantitative feed restriction increased lipogenic and decreased lipolytic gene expression. In the refeeding period, <jats:italic>CPT1\u2010A<\/jats:italic> gene expression was induced, without changing the broilers body composition. Positive associations were found between BWG (Body Weight Gain) and <jats:italic>Lactobacillus<\/jats:italic> and <jats:italic>Clostridium<\/jats:italic> cluster IV groups, and negatively associations with <jats:italic>Enterobacteriaceae<\/jats:italic> and <jats:italic>Enterococcus<\/jats:italic> bacterial groups. In conclusion, differences found in microbiota were similar between the two feed restriction programmes, however, hepatic gene expression differences were only found in quantitative restriction. Higher counts of <jats:italic>Lactobacillus <\/jats:italic>and <jats:italic>Clostridium<\/jats:italic> cluster IV groups in ileum are likely to be related to better broiler performance and low expression of lipogenic genes.<\/jats:p>","DOI":"10.1111\/jpn.13093","type":"journal-article","created":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T16:45:01Z","timestamp":1554137101000},"page":"1070-1080","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Intestinal microbiota of broilers submitted to feeding restriction and its relationship to hepatic metabolism and fat mass: Fast\u2010growing strain"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-4442","authenticated-orcid":false,"given":"Raquel","family":"Lunedo","sequence":"first","affiliation":[{"name":"School of Agricultural and Veterinary Sciences S\u00e3o Paulo State University (Unesp) Jaboticabal, S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz R.","family":"Furlan","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (Unesp), Aquaculture Center of Unesp Jaboticabal, S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel F.","family":"Fernandez\u2010Alarcon","sequence":"additional","affiliation":[{"name":"School of Agricultural and Veterinary Sciences S\u00e3o Paulo State University (Unesp) Jaboticabal, S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo H.","family":"Squassoni","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (Unesp), Aquaculture Center of Unesp Jaboticabal, S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel M. 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