{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:15:10Z","timestamp":1786580110471,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T00:00:00Z","timestamp":1555459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Eatlive Industrial Co. LTD"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was conducted to examine the effects of weaning stress on gene expression of specific markers in hypothalamus\u2010pituitary\u2010adrenal (HPA) axis and neuronal activity in the newly weaned piglets. Twelve 28\u2010days\u2010old, newly weaned crossbred (Landrace\u00a0\u00d7\u00a0Yorkshire\u00a0\u00d7\u00a0Duroc) male piglets from 6\u00a0l (2\u00a0piglets\/l) were randomly categorized into two groups: (a) weaning stress: piglets were separated from their dams, relocated and mixed with the unacquainted domestic piglets for 2\u00a0hr (stress, <jats:italic>n<\/jats:italic>\u00a0=\u00a06); (b) no\u2010stress: piglets stayed with their dams in the farrowing house (NS; <jats:italic>n<\/jats:italic>\u00a0=\u00a06). After weaning stress, all piglets were electrically euthanized and the blood samples\/HPA tissues were collected for subsequent analysis, including plasma cortisol and mRNA expression of c\u2010fos, c\u2010jun, corticotropin\u2010releasing hormone (CRH), CRH receptor 1 (CRHR\u20101) and adrenocorticotropin hormone receptor (MC2R). Results: Weaning stress significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) increased the plasma cortisol level and suppressed the expression of c\u2010fos and CRH in hypothalamus. In addition, weaning stress enhanced the mRNA abundance of c\u2010jun and CRHR\u20101 in the pituitary gland. No significant differences in the gene expression of MC2R and CRHR\u20101 were observed in the adrenal gland between treatment groups. Taken together, HPA involved in weaning stress and CRHR\u20101 and c\u2010jun could be potential markers to evaluate the activation of HPA axis.<\/jats:p>","DOI":"10.1111\/jpn.13104","type":"journal-article","created":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T10:55:10Z","timestamp":1555498510000},"page":"1218-1223","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The immediate effects of weaning stress on the hypothalamus\u2010pituitary\u2010adrenal alteration of newly weaned piglets"],"prefix":"10.1111","volume":"103","author":[{"given":"Chien\u2010Ho","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine National Chiayi University Chiayi Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6281-0007","authenticated-orcid":false,"given":"Chih\u2010Cheng","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine National Chiayi University Chiayi Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1591(96)01084-2"},{"issue":"4","key":"e_1_2_7_3_1","first-page":"366","article-title":"The impact of handling and environmental\u2010factors on the stress response and its consequences in swine","volume":"36","author":"Barnett J. 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