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Alternative source of NPN is the slow release of ammonia sources in which the product is the extrusion of starch with urea. This work aimed to determine the effects on nutrient intake, ingestive behaviour, digestibility, nitrogen balance, ruminal pH, rumen ammonia nitrogen, volatile fatty acids (VFA) and blood parameters with increased levels of extruded urea (50, 60, 70 and 80\u00a0g\/100\u00a0kg of body weight [BW]) in beef cattle diet. Four rumen cannulated crossbred steers with initial mean weight of 336\u00a0\u00b1\u00a047\u00a0kg in a 4\u00a0\u00d7\u00a04 Latin square design were distributed. Diets were formulated with 400:600\u00a0g\/kg roughage:concentrate ratio on dry matter based and provided once per day, being used whole corn silage as roughage. There were no effects on nutrient intake (kg\/day), ingestive behaviour, apparent digestibility, nitrogen balance, volatile fatty acid (VFA) and blood parameters in extruded urea treatment groups. Similar results were observed on time spent on feeding, rumination and idleness. There were positive linear effects (<jats:italic>p\u00a0=\u00a0<\/jats:italic>0.022) on rumen pH in the time of 8\u00a0hr after feeding and also on plasma concentration of the extruded urea levels (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.039); whereas a linear negative effect (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.030) was observed on ammonia nitrogen for the collection time of 2\u00a0hr after feeding. Increased levels of extruded urea could maintain nutrient intake, digestibility, ingestive behaviour, rumen pH and blood parameters in normal conditions. In conclusion, we recommend the extruded urea use with values up to 80\u00a0g\/100\u00a0kg BW in confined beef cattle that receive balanced diets with 140\u00a0g\/kg of crude protein.<\/jats:p>","DOI":"10.1111\/jpn.13140","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T10:49:27Z","timestamp":1560250167000},"page":"1283-1294","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Extruded urea could reduce true protein source in beef cattle diets"],"prefix":"10.1111","volume":"103","author":[{"given":"Gabriella J.","family":"de Moraes","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science Federal University of Mato Grosso do Sul Campo Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6895-8483","authenticated-orcid":false,"given":"Lu\u00eds Carlos V.","family":"\u00cdtavo","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science Federal University of Mato Grosso do Sul Campo Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4790-8177","authenticated-orcid":false,"given":"Camila Celeste B. 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