{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T12:29:29Z","timestamp":1766579369286,"version":"3.40.5"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T00:00:00Z","timestamp":1573084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Various feeding studies have been conducted with the different species of animals to evaluate the possible transfer of transgenic DNA (tDNA) from genetically modified (GM) feed into the animal tissues. However, the conclusions drawn from most of such studies are sometimes controversial. Thus, in the present study, an attempt has been made to evaluate the fate of tDNA in rabbits raised on GM cotton\u2010based diet through PCR analysis of the DNA extracted specifically from blood, liver, kidney, heart and intestine (jejunum). A total of 48 rabbits were fed a mixed diet consisting variable proportions of transgenic cottonseeds meal (i.e. 0% w\/w, 20% w\/w, 30% w\/w and 40% w\/w) for 180\u00a0days. The presence of transgenic DNA fragments (<jats:italic>Cry1Ac, Cry2A<\/jats:italic> and <jats:italic>CP4 EPSPS<\/jats:italic>) or plant endogenous gene (<jats:italic>Sad1<\/jats:italic>) was traced in those specific tissues and organs. The presence of \u03b2\u2010actin (<jats:italic>ACTB<\/jats:italic>) was also monitored as an internal control. Neither the transgenic fragments (459\u00a0bp of <jats:italic>Cry1Ac<\/jats:italic> gene, 167\u00a0bp of <jats:italic>Cry2A<\/jats:italic> gene and111 bp of <jats:italic>CP4 EPSPS<\/jats:italic> gene) nor cotton endogenous reference gene (155\u00a0bp of <jats:italic>Sad1)<\/jats:italic> could be detected in any of the DNA samples extracted from the rabbit's tissues in both control and transgenic groups. However, 155\u00a0bp fragment of the rabbit's reference gene (<jats:italic>ACTB<\/jats:italic>) was recovered in all the DNA samples extracted from rabbit tissues. The results obtained from this study revealed that both plant endogenous and transgenic DNA fragments have same fate in rabbit's tissues and were efficiently degraded in the gastrointestinal tract (GIT).<\/jats:p>","DOI":"10.1111\/jpn.13243","type":"journal-article","created":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T04:15:41Z","timestamp":1573186541000},"page":"343-351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessing the fate of recombinant plant DNA in rabbit\u2019s tissues fed genetically modified cotton"],"prefix":"10.1111","volume":"104","author":[{"given":"Ahmad Ali","family":"Shahid","sequence":"first","affiliation":[{"name":"Centre of Excellence in Molecular Biology University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7545-6641","authenticated-orcid":false,"given":"Ibrahim Bala","family":"Salisu","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology University of the Punjab Lahore Pakistan"},{"name":"Department of Animal Science Faculty of Agriculture Federal University Dutse Dutse Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amina","family":"Yaqoob","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul Qayyum","family":"Rao","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inayat","family":"Ullah","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tayyab","family":"husnain","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Molecular Biology University of the Punjab Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2006.08.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2004.04.026"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2019.01.010"},{"key":"e_1_2_8_5_1","first-page":"197","article-title":"Safety issues associated with the DNA in animal feed derived from genetically modified crops. 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