{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T20:37:15Z","timestamp":1775939835269,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,24]],"date-time":"2019-11-24T00:00:00Z","timestamp":1574553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate the effects of different levels of enzymatic hydrolysate of dietary locust bean gum on nutrient digestibility, intestinal morphology and microflora of broilers, a total of 768 one\u2010day\u2010old Arbor Acres (AA) broiler chicks were randomly divided into 6 treatments with 8 replicates per treatment and 16 birds per replicate. The treatments were as follows: (1) CON, basal diet; (2) ANT, basal diet +62.5\u00a0mg\/kg flavomycin; (3) LBG, basal diet +0.1% locust bean gum; (4) ELBG\u20100.1, basal diet +0.1% enzymatic hydrolysate of LBG; (5) ELBG\u20100.2, basal diet +0.2% enzymatic hydrolysate of LBG; and (6) ELBG\u20100.3, basal diet +0.3% enzymatic hydrolysate of LBG. The digestibilities of ether extract, crude protein and dry matter were increased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) in broilers fed the ELBG\u20100.3 diet compared with the CON and LBG diets on day 21. Duodenal villus height and the ratio of the villus height to crypt depth were greater (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) in broilers fed the ELBG\u20100.3 diet than the CON, ANT and LBG diets. Jejunum villus height was higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in broilers fed the ELBG\u20100.2 and ELBG\u20100.3 diets than the CON diet. The number of caecal <jats:italic>Escherichia coli<\/jats:italic> was reduced (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.01) in broilers fed the ELBG\u20100.2 and ELBG\u20100.3 diets compared with the CON diet. The number of caecal <jats:italic>Lactobacilli<\/jats:italic> was greater (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in broilers fed the ELBG\u20100.3 diet than the CON and ANT diets. In summary, the addition of 0.3% locust bean enzymatic hydrolysate can increase the surface area of intestinal villi and the number of beneficial bacteria, inhibit the proliferation of harmful bacteria, maintain the balance of intestinal microflora and improve the digestibility of nutrients.<\/jats:p>","DOI":"10.1111\/jpn.13245","type":"journal-article","created":{"date-parts":[[2019,11,25]],"date-time":"2019-11-25T05:03:10Z","timestamp":1574658190000},"page":"230-236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effects of enzymatic hydrolysate of locust bean gum on digestibility, intestinal morphology and microflora of broilers"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9083-1259","authenticated-orcid":false,"given":"Jingjing","family":"Xie","sequence":"first","affiliation":[{"name":"National Engineering Research Center of Biological Feed Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Institute of Feed Research Chinese Academy of Agriculture Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8784-2205","authenticated-orcid":false,"given":"Zhongcheng","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Biological Feed Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Institute of Feed Research Chinese Academy of Agriculture Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3688-5413","authenticated-orcid":false,"given":"Hu","family":"Cui","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Biological Feed Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Institute of Feed Research Chinese Academy of Agriculture Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Nie","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Biological Feed Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Institute of Feed Research Chinese Academy of Agriculture Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tietao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Special Animal and Plant Sciences Chinese Academy of Agricultural Sciences Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuhua","family":"Gao","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Biological Feed Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Institute of Feed Research Chinese Academy of Agriculture Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Qiao","sequence":"additional","affiliation":[{"name":"National Engineering Research Center of Biological Feed Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Institute of Feed Research Chinese Academy of Agriculture Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,24]]},"reference":[{"issue":"1","key":"e_1_2_7_2_1","first-page":"167","article-title":"Evaluation of fructooligosaccharides on performance and carcass yield of male broilers","volume":"68","author":"Ammerman E.","year":"1989","journal-title":"Poultry Science"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0308-8146(93)90152-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071669608417845"},{"key":"e_1_2_7_5_1","first-page":"1298","volume-title":"Official methods of analysis","author":"AOAC","year":"1990"},{"issue":"1","key":"e_1_2_7_6_1","first-page":"368","article-title":"\u03b2-Galactomannan and Saccharomyces cerevisiae var. boulardii","volume":"19","author":"Badia R.","year":"2013","journal-title":"Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/86.6.1070"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-7219"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2007-00249"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1079\/9780851993935.0145","volume-title":"Non-starch Polysaccharides: Effect on nutritive value in Enzymes in farm animal nutrition","author":"Choct M. 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