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Considering the beneficial properties of L\u2010arginine on gut function, we investigated the protective effects of L\u2010arginine on the intestine under HS conditions. In vivo, the serum cortisol level and the rectal temperature increased in response to HS. Under HS, the intestinal damage showed obvious morphological changes. Furthermore, HS decreased the mRNA and protein expression levels of <jats:italic>Nurr1<\/jats:italic>, <jats:italic>ZO\u20101<\/jats:italic>, <jats:italic>occludin<\/jats:italic>, <jats:italic>claudin\u20106<\/jats:italic> and <jats:italic>E\u2010cadherin<\/jats:italic>, increased the mRNA expression of <jats:italic>NF\u2010\u03baB<\/jats:italic> and <jats:italic>IL\u20101\u03b2<\/jats:italic>, and increased the protein expression of cleaved caspase\u20103. In contrast, L\u2010arginine supplementation maintained intestinal integrity and increased the villus\/crypt ratio. L\u2010arginine also suppressed the expression of inflammation\u2010related genes and the protein expression of cleaved caspase\u20103, whereas it upregulated the mRNA and protein expression of tight junction proteins and LC3B protein expression. In vitro, L\u2010arginine attenuated HS\u2010induced apoptosis as demonstrated by flow cytometry and decreased cleaved caspase\u20103 protein expression. L\u2010arginine induced autophagy, which was demonstrated by decreased expression of p62 and p\u2010mTOR\/mTOR, and increased expression of LC3B. The protein expression levels of TJ proteins also enhanced by L\u2010arginine in IEC\u20106 cells. Taken together, these results suggest that L\u2010arginine can alleviate intestinal damage and protect the intestinal integrity by suppressing local inflammation response, promoting the production of TJs and facilitating autophagy under HS conditions.<\/jats:p>","DOI":"10.1111\/jpn.13246","type":"journal-article","created":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T09:58:38Z","timestamp":1573466318000},"page":"385-396","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Protective effects of L\u2010arginine on the intestinal epithelial barrier under heat stress conditions in rats and IEC\u20106 cell line"],"prefix":"10.1111","volume":"104","author":[{"given":"Liqing","family":"Huang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine China Agricultural University Beijing 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