{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T16:58:37Z","timestamp":1784912317298,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T00:00:00Z","timestamp":1575504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Key Program of the National Natural Science Foundation of China","award":["31630073"],"award-info":[{"award-number":["31630073"]}]},{"name":"Special Funds of CAAS for Distinguished Scientists"},{"name":"National Key R&D Program of China","award":["2017YFD0502200"],"award-info":[{"award-number":["2017YFD0502200"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31472116"],"award-info":[{"award-number":["31472116"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"China Agriculture Research System","award":["CARS-41"],"award-info":[{"award-number":["CARS-41"]}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"crossref","award":["ASTIP-IAS09"],"award-info":[{"award-number":["ASTIP-IAS09"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two experiments were conducted to investigate the kinetics of phosphorus (P) absorption and expressions of type IIb sodium\u2010dependent\u00a0phosphate cotransporter (NaP\u2010IIb), inorganic phosphate transporters 1 and 2 (PiT\u20101 and PiT\u20102) in primary cultured duodenal epithelial cells of chick embryos. In experiment 1, the P absorptions across duodenal epithelial cell monolayers at different incubation time points (0, 10, 20, 40, 60, 80, 100 and 120\u00a0min) were compared. In experiment 2, the kinetics of P absorption was performed at 40\u00a0min after incubation of duodenal epithelial cells with the media containing 0, 0.75, 1.5, 3.0, 6.0, 12.0, 24.0 and 48.0\u00a0mmol P\/L as KH<jats:sub>2<\/jats:sub>PO<jats:sub>4<\/jats:sub>, and the mRNA and protein expression levels of NaP\u2010IIb, PiT\u20101 and PiT\u20102 in duodenal epithelial cells with the media containing 0, 6.0 and 48.0\u00a0mmol P\/L were determined at 87\u00a0min after incubation. The results from experiment 1 showed that the P absorption increased linearly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.0001) from 0 to 80\u00a0min and the fastest increase occurred at 40\u00a0min; the asymptotic model was shown to have the best fit degree, and the optimal incubation time for saturable P absorption was determined to be 87\u00a0min. The kinetic curves of P absorption from experiment 2 demonstrated that P absorption was a mixed process of a non\u2010saturable diffusion plus a saturable carrier\u2010mediated transport across the duodenal epithelial cells. The high P concentration (48.0\u00a0mmol\/L) decreased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) NaP\u2010IIb and PiT\u20101 mRNA and protein levels and increased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.0001) PiT\u20102 mRNA level. These results indicated that the P absorption across primary cultured duodenal epithelial cell monolayers of chick embryos was a mixed process of a non\u2010saturable diffusion plus a saturable carrier\u2010mediated transport and could be restricted by reducing the NaP\u2010IIb and PiT\u20101 expressions while increasing the PiT\u20102 expression at a high P concentration.<\/jats:p>","DOI":"10.1111\/jpn.13260","type":"journal-article","created":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T06:04:23Z","timestamp":1575612263000},"page":"237-244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Kinetics of phosphorus absorption and expressions of related transporters in primary cultured duodenal epithelial cells of chick embryos"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6302-1448","authenticated-orcid":false,"given":"Sumei","family":"Cao","sequence":"first","affiliation":[{"name":"Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing P. R. China"},{"name":"Laboratory of Feed Biotechnology State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5428-1720","authenticated-orcid":false,"given":"Guoqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Lu","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Feed Biotechnology State Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiudong","family":"Liao","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xugang","family":"Luo","sequence":"additional","affiliation":[{"name":"Mineral Nutrition Research Division Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.4.C1135"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00034.2008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.3.457"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00588690"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/19.4.411"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2133\/dmpk.21.217"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2012.12187"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(79)80368-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.22237\/jmasm\/1036110180"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00279.2009"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114518000934"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.72351"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-013-9670-y"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pew034"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/sky434"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2016-0430"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-012-9332-x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2011-02061"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5729"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-3119(17)61701-5"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-203-43607"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-1891-4-34"},{"key":"e_1_2_7_25_1","volume-title":"Nutrient requirements of poultry","author":"NRC","year":"1994"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-135280-6.50036-7"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1978.234.4.E379"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-3119(17)61704-0"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/103.4.586"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2016-0713"},{"key":"e_1_2_7_31_1","first-page":"3159","article-title":"Isolation and identification of duodenal epithelial cells of broiler embryos in vitro and its establishment and evaluation of primary cultured absorption model","volume":"30","author":"Zhang S. M.","year":"2018","journal-title":"Chinese Journal of Animal Nutrition"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114515003803"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.13260","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13260","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpn.13260","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13260","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T12:07:44Z","timestamp":1694088464000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.13260"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,5]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/jpn.13260"],"URL":"https:\/\/doi.org\/10.1111\/jpn.13260","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,5]]},"assertion":[{"value":"2019-07-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}