{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:05:40Z","timestamp":1758632740104,"version":"3.40.5"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T00:00:00Z","timestamp":1580169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Riddet Institute"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to evaluate the relationship among the number of bacteria, number of goblet cells, gut mucin gene expression, mucin protein and immunity protein levels of rats fed a diet containing freeze\u2010dried ovine Ig (FD). Sprague Dawley male rats were used in a 21\u2010days study and were fed a basal control diet (BD; no Ig) and a test diet containing freeze\u2010dried ovine Ig (FD). Diets were isocaloric and contained the same amount of the first limiting amino acids, methionine plus cysteine. Pearson's correlation analysis was conducted on the data (stomach, ileum and colon) obtained from individual rats (<jats:italic>n<\/jats:italic>\u00a0=\u00a010) fed either casein\u2010based diet (BD) or ovine serum Ig (FD) to evaluate the relationship between number of bacteria, number of goblet cells, gut mucin gene expression and gut mucin protein levels. Pearson's correlation analysis was then conducted with the data from the FD fed rats to evaluate the relationship among the above said variables. In the stomach content, a significant (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) correlation was found between the Muc5Ac gene expression and mucosal mucin protein. In the ileum and colon, a significant (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) correlation was observed among the mRNA levels of mucin (Muc2 and Muc4) genes. There was also evidence of a strong relationship (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) between digesta mucin and mucosal mucin protein concentrations. A negative correlation of mucosal IgA protein concentration with total<jats:italic>Lactobacillus<\/jats:italic>(in ileum and colon) and total bacteria (in the ileum) was not evident with FD fed rats when compared to the results obtained using both BD and FD fed rats. In conclusion, this study suggests that feeding freeze\u2010dried ovine Ig in growing rats results in a strong correlation between the number of bacteria, mucin and immunity proteins.<\/jats:p>","DOI":"10.1111\/jpn.13319","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T07:35:32Z","timestamp":1580283332000},"page":"758-766","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dietary supplementation with ovine serum immunoglobulin modulates correlations between mucin, microbiota and immunity proteins in the growing rat"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0540-1471","authenticated-orcid":false,"given":"Prabhu","family":"Balan","sequence":"first","affiliation":[{"name":"Riddet Institute Massey University Palmerston North New Zealand"},{"name":"Alpha Massey Natural Nutraceutical Research Centre Palmerston North New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryann","family":"Staincliffe","sequence":"additional","affiliation":[{"name":"AgResearch Hamilton New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul J.","family":"Moughan","sequence":"additional","affiliation":[{"name":"Riddet Institute Massey University Palmerston North New Zealand"},{"name":"Alpha Massey Natural Nutraceutical Research Centre Palmerston North New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjsurg.2006.11.026"},{"volume-title":"Effects of orally administered ovine serum immunoglobulin in the normal and Salmonella enteritidis \u2010 challenged growing rat","year":"2011","author":"Balan P.","key":"e_1_2_8_3_1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.12060"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-6028"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.108.099630"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731111000620"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731110000868"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.131433"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731111001108"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114513000172"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13258"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.46.2.218"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00265.2006"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00535.2001"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12820"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"439865","DOI":"10.1155\/2013\/439865","article-title":"Goblet cells and mucin related gene expression in mice infected with Eimeria papillata","volume":"125","author":"Dkhil M. A.","year":"2013","journal-title":"The Scientific World Journal"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00428.2002"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.003101"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.7.1653"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4fo00087k"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2011.03012.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-008-8352-7"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf800080e"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.3.C351"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2006.0132"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2007.02.001"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2008.04.001"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511003655"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00090.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394294-4.00009-2"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2012.02.013"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.2527\/2005.83122876x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3109\/09637489609006952"},{"volume-title":"R: A language and environment for statistical computing","year":"2013","author":"R Core Team","key":"e_1_2_8_35_1"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.23383"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1985.248.4.G418"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.1.70"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00391"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-8-19"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.4137\/CGast.S13200"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.13319","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13319","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpn.13319","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13319","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T20:38:14Z","timestamp":1722371894000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.13319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,28]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1111\/jpn.13319"],"URL":"https:\/\/doi.org\/10.1111\/jpn.13319","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"type":"print","value":"0931-2439"},{"type":"electronic","value":"1439-0396"}],"subject":[],"published":{"date-parts":[[2020,1,28]]},"assertion":[{"value":"2019-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}