{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T18:37:51Z","timestamp":1788633471911,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T00:00:00Z","timestamp":1583280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003121","name":"Ferdowsi University of Mashhad","doi-asserted-by":"publisher","award":["3\/41461"],"award-info":[{"award-number":["3\/41461"]}],"id":[{"id":"10.13039\/501100003121","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003121","name":"Ferdowsi University of Mashhad","doi-asserted-by":"publisher","award":["7\/23\/2016"],"award-info":[{"award-number":["7\/23\/2016"]}],"id":[{"id":"10.13039\/501100003121","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The availability of plant phosphorus in the gut chicken can be improved by increasing phosphorus retention using phytase enzyme or a probiotic with phytase activity as an alternative. In this study, the efficacy of a recombinant probiotic, <jats:italic>Lactococcus lactis<\/jats:italic> (<jats:italic>L.\u00a0lactis<\/jats:italic>), with a potential of phytase production was evaluated in broiler chickens. To this aim, 360 one\u2010day\u2010old male broiler Cobb 500 were divided into six treatments with six replicates and reared to 42\u00a0days of age. The experimental treatments included positive control diet containing adequate phosphorus (PC), negative control diet containing reduced available phosphorus (NC), negative control diet involving recombinant <jats:italic>L.\u00a0lactis<\/jats:italic> (RLL), negative control diet containing both recombinant <jats:italic>L.\u00a0lactis<\/jats:italic> and <jats:italic>Lactobacillus salivarius<\/jats:italic> (RLL\u00a0+\u00a0LBS), negative control diet including non\u2010recombinant <jats:italic>L.\u00a0lactis<\/jats:italic> (LL) and negative control diet containing Hostazym<jats:sup>\u00ae<\/jats:sup>. Growth performance, total tract apparent disappearance of phytate\u2010P and nutrient retention, mineral content of the tibia and histomorphology of jejunum were evaluated at the age of 35\u00a0days. Based on the results, the phosphorus (P) deficiency in the diet reduced body weight (BW), average daily gain (ADG), length and strength of tibia and increased feed conversion ratio (FCR) compared to PC group. However, the supplementation of Hostazym<jats:sup>\u00ae<\/jats:sup> or RLL probiotic into the feed improved BW, ADG, FCR, disappearance of Phytate\u2010P and retention of P, length and strength of the tibia in a level similar to PC treatment. Phosphorus content of tibia in the chickens fed P\u2010deficient diets containing RLL was similar to that of the tibia in the control group. Excreta phytate and total P excretion of the chickens decreased when diets contained Hostazym<jats:sup>\u00ae<\/jats:sup>, RLL and RLL\u00a0+\u00a0LBS. In addition, the diet containing RLL\u00a0+\u00a0LBS probiotic increased villi height compared with other treatments (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Further, recombinant <jats:italic>L.\u00a0lactis<\/jats:italic> could represent phytase activity in the gut environment of the chickens and could be an alternative to the commercial phytase.<\/jats:p>","DOI":"10.1111\/jpn.13332","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T09:16:31Z","timestamp":1583313391000},"page":"909-917","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Performance of an <i>Escherichia coli<\/i> phytase expressed in <i>Lactococcus lactis<\/i> on nutrient retention, bone traits and intestinal morphology in broiler chickens"],"prefix":"10.1111","volume":"104","author":[{"given":"Masoud","family":"Mohammadi Ziarat","sequence":"first","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture Ferdowsi University of Mashhad Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan","family":"Kermanshahi","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture Ferdowsi University of Mashhad Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan","family":"Nasiri Mogaddam","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture Ferdowsi University of Mashhad Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1948-9734","authenticated-orcid":false,"given":"Reza","family":"Majidzadeh Heravi","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture Ferdowsi University of Mashhad Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,4]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Official methods of analysis","author":"AOAC","year":"1990"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aqrep.2017.12.003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03155-13"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2011.160.168"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0377-8401(03)00207-4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.22358\/jafs\/66142\/2012"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.8.1389"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19790074"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.6998"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/84.10.1616"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.11.1939"},{"issue":"1","key":"e_1_2_9_13_1","first-page":"59","article-title":"Phytic acid content of cereals and legumes and interaction with proteins","volume":"46","author":"H\u00eddv\u00e9gi M.","year":"2002","journal-title":"Periodica Polytechnica Chemical Engineering"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2012-02528"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60038a038"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.22.4.429"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071660701485034"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJMR11.416"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1075762"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071668.2013.850469"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.17221\/2992-VETMED"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.6365"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2009-00308"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0043933907001663"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-12163-3"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-018-9423-x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.10.1737"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0750069"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071660050022263"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2007-00247"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/85.1.82"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0308-8146(94)90109-0"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2011-01702"},{"key":"e_1_2_9_34_1","unstructured":"SAS Institute. 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